IMPACT OF NORMAL AND PATHOLOGICAL ACTIVITIES ON KCC2 MOBILITY AND FUNCTION IN
HIPPOCAMPAL SLICES
The K+/Cl? cotransporter KCC2, which extrudes
intracellular chloride controls chloride
homeostasis that critically
determines the polarity and efficacy of GABAergic transmission in the
brain.
Our focus is to understand the mechanisms that control KCC2 membrane
expression and
function in order to understand ?and possibly interact with?
the functional consequences of a brain
trauma or epileptic activity in the
adult brain.
The proposed project is to study the membrane dynamics of the
KCC2 transporter in
integrated network of cortical neurons, and to examine
how these properties can be modulated
under normal and pathological epileptic
conditions. This project will require developing the Single
Particle Tracking
technique in brain slices. For this purpose, the post?doctoral candidate will
collaborate with biochemists and specialists in biomedical imaging to develop
Single Particle Tracking
technique in an integrated neuronal network.
The lab
is located in the heart of Paris in a rich neuro?scientific environment. The
IFM houses
state of the art research facilities (bi?photon microscope,
several confocal microscopes for fixed
preparation and live cell imaging,
electrophysiology rigs, single particle tracking rigs coupled with
electrophysiology, ratiometric fluorescence and photolysis system as well as
molecular biology and
imaging platforms).
Preference will be given to
candidates with a track record of peer?reviewed publications,
extensive
experience with cell imaging. Please email application with CV, publications,
contact
information of two references, and statement of research interests to
Sabine L.vi
(sabine.levi@inserm.fr).
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