3)Would your phospho-sumoylated proteins likely to be also O-glycosylated
and/or N-glycosylated ? Why? How may ETD or ECD help to identify and
distinguish site-specific Ser/Thr O-glycosylation from phosphorylation,
as compared with normal CID MS/MS ?
Answer :
O-glycosylation and N-glycosylation both not. Because both
O-or N- glycosylated proteins are synthesized and degraded
on membrane or surround by membrane. And these protein don't
have chance to be sumoylated (occurs in cytoplasm or nuclear
plasma )
fragmentation cause by ECD and ETD is fast and specific and
labile PTM (phosphorylation of Ser/Thr and glycosylation)
and non-covalent bonds often remain intact after backbone bond
dissociation. But labile PTM usually loss by CID. Therefore
we can distinguish site specific glycosylation or phosphorylation
by ECD (or ETD).
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