※ 引述《ndmcls.bbs@ptt.cc (季勗)》之銘言：
> 好啊, 我誠心誠意的請教你怎麼轉的, 我也
> 想用, 因為這樣的效率會更高.
> 用載體效率低喔.
1. make up the construct with promoter, coding ORF, and terminator
2. bombard it into the cell
3. grow on selective media
Is this what u want to know? I am not sure about ur question.
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