※ 引述《kukukukukuku.bbs@ptt.cc (拉拉:您好 掰掰)》之銘言：
> 這是題目
> Q:The size of Okazaki fragments differs in different organisms.
> What might influence this size? How might you test this possibility?
> 這是找的答案 我有努力翻過,但是還是看的哩哩落落/_\ 真的是無計可施
> 所以來請教各位大大,字數很多 真對不住~ 不用全翻 只跪求重點<(_ _)>
> Ans:
> The size of Okazaki fragments in a given organism reflects the specific
> kinetics governing the association between primase and the other replication
> components during lagging strand synthesis in the cell. Primase only joins
> the replication fork periodically, sticking around just long enough to
> synthesize an RNA primer and thereby trigger lagging strand synthesis.
> The frequency with which primase joins the fork can differ between organisms,
> leading to characteristic differences in the length of the Okazaki fragments.
> In organisms where primase has a high affinity for the other replication
> factors (such as helicase), for example, the enzyme will associate with the
> fork more frequently, resulting in shorter Okazaki fragments. Alternatively,
> when the primase has less affinity for the fork, it will associate less
> frequently, leading to longer Okazaki fragments.
岡崎片段的長度與複製叉對primase吸引力成反比，
吸引力強代表primase在複製叉附近的consentration高
所以okazaki fragments會比較短
> You could experimentally address the role of primase and its association with
> the replication fork in determining Okazaki fragment length by altering the
> association and seeing what effect this has on fragment length. For example,
> you could increase or decrease the concentration of primase in a cell to see
> if higher concentrations (which should lead to more frequent primase-fork
> interactions) result in shorter Okazaki fragments, and if lower concentrations
> lead to longer ones. You could also isolate mutant forms of primase that have
> higher or lower affinities for helicase (or other replication components) and
> ask whether the Okazaki fragment length is altered in the mutant cells.
控制primase之consentration，觀察okazaki fragment
控制法有1.增加primase濃度 2.增加helicase與primase之親合力
請指正^^"
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