推 bluecsky:跟自體螢光相比後轉換的? 12/01 16:40
A TF-1 cell proliferation assay was used to assess the bioactivity of GMCSF–
GFP. GMCSF–GFP maintains nearly 80% of the bioactivity of commercially
produced GMCSF. GMCSF–GFP bioactivity. Indicated concentrations of GMCSF–
GFP and a GMCSF standard were added to cultures of cytokine-dependent cell line
TF-1. Cell proliferation was measured by OD 490 nm.
使用OD 490 nm測定的GMCSF–GFP bioactivity.
問題在於nearly 80%,paper上是因GMCSF–GFP及GMCSF濃度越高,OD 490 nm測的數值也
越高,但老師要知道那80%是以什麼為基準的?
謝謝! 我一直認為那是吸光值給的數值0.8轉成百分比
但老師說一定有個基準,不可能無故轉換成百分比!!
本人愚昧阿!!(一個頭兩個大阿!!)
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平衡就是不斷的循環 ○→●→□→■→△→▲→○ (  ̄ c ̄)y▂ξ
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