推 apa9394:This is what I want to say... thank you, mouse king^^y 10/18 17:05
※ 引述《kate1114 (斑點蘭獻給迷迭香)》之銘言：
: 最近報告 常聽到什麼''利用cDNA cloning 找出了一段miRNA...blablabla
: ...""
: 但之前上課又學到說 cDNA clone技術的應用:
: 就是用由sample抽來的protein-->知此protein mRNA sequence-->再合成(還是反轉錄)cDNA
: (mature form)
(1)這一步是天方夜譚，聽過wobble hypothesis嗎？你這邏輯應該是理論吧！
: ----->再從genome比對--->可找出此protein可能有其他沒被偵測的(pre-mature form)
(2)protein mature與否是由translational modification決定和genome sequence 無關喔！
: 我不知道我有沒有會錯老師的意思 不過依照我上課寫的筆記
: 要解釋'利用cDNA cloning 找出了一段miRNA"真的很奇怪 > <
: 有大大知道如何解釋咩~~
: 要是我上面哪裡寫錯了 也歡迎大家指正
: 謝謝^^
(3) 你這裡的miRNA用的有點怪（miRNA的來源和定義見下文），目前cDNA的製備多採用
RT-PCR的方式，很少有人是從protein下手，因為目前protein序列分析技術沒有核酸序
列分析來的方便；miRNA也絕對不是從cDNA cloning找的到的！
In genetics, microRNAs (miRNA) are single-stranded RNA molecules of about 21-23
nucleotides in length thought to regulate the expression of other genes.
miRNAs are encoded by RNA genes that are transcribed from RNA but not trans-
lated into protein; instead they are processed from primary transcripts known
as pri-miRNA to short stem-loop structures called pre-miRNA and finally to
functional miRNA. Mature miRNA molecules are complementary to regions in one
or more messenger RNA (mRNA) molecules, which they target for degradation
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