※ 引述《georgeby (和平 奮鬥 救雞排)》之銘言：
: Ct值(threshold cycle number)
: 請問是PCR循環的次數嗎?
: 那怎麼決定Ct值
: 是做到我們要的那段為止嗎?
: 謝謝
這是英文的定義
The concept of the threshold cycle (Ct) allows for accurate
and reproducible quantification using fluorescence based
RT-PCR. Fluorescene values are recorded during every cycle
and represent the amount of product amplified to that point
in the amplification reaction. The more templates present at
the beginning of the reaction, the fewer number of cycles it
takes to reach a point in which the fluoresent signal is first
recorded as statistically significant above background, which
is the definition of the (Ct) values.
有看沒有懂？根據我去Roche上課的記憶.....應該是曲線一次微分（即斜率）
的最大值.....因為軟體會幫你算....所以很多人都只知道收data卻不知原理
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