各為先進好
最近在看~~~一篇chromosone 22q11.2 deletion的paper
網路上查了一下FISH
utilizingfluorescently labeled DNA probes
to detect or confirm gene or chromosome abnormalities
1.first denatured, a process that separates the complimentary strands
within the DNA double helix structure
2.fluorescently labeled probe of interest is then added to the denatured
sample mixture and hybridizes with the sample DNA at the target site
3.probe signal can then be seen through a fluorescent microscope and
the sample DNA scored for the presence or absence of
the signal.
我的問題是....
1.如果我們只懷疑他有chromosome
但不知道那一對的話.....要如果detect出那一對有問題呢
2.像DiGeroge syndrome如果11q11.2 deletion...它都deletion了如何insert probe
3.像DiGeroge syndrome它的probe要如何選擇呢~~怎麼知道那一斷的核酸排列阿
11q11.2 那一段是啥?
謝謝
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