Re: 請問有關lentivirus vector

[lenti (啄木鳥)]

※ 引述《allergy1115 (白天彼此明爭暗鬥)》之銘言：
: 最近確定我要改用lentivirus vector tet on/off system
: 找了invitrogen, 不太確定這家有這套系統嗎,還是我眼殘@@"
: 請高手幫忙解惑一下謝謝...!
: ps 除了Neo 是用G418在篩選細胞, 還有哪種gene(marker)
:    也是用G418在篩穩定clone的呢？

TronoLab 提供.

直接到Addgene 網站申請, http://www.addgene.org/pgvec1

只需付運費.

pLVTHM
http://tronolab.epfl.ch/webdav/site/tronolab/shared/control/plvthm.pdf

pLV-tTRKRAB
http://tronolab.epfl.ch/webdav/site/tronolab/shared/control/plvttrkrab.pdf

pLV-tTRKRAB-Red
http://tronolab.epfl.ch/webdav/site/tronolab/shared/control/plvttrkrabred.pdf

The target cells need to be cotransduced with both pLVTHM and pLV-tTRKRAB-Red
to achieve conditional expression of siRNA. However pLVTHM alone can be used
for constitutive expression of siRNA.


Open Biosystem   探索科技代理

這家的lentivector 都不錯, 但我沒有用過Tet on/off system.

TRIPZ lentiviral inducible shRNAmir
for tightly regulatable RNAi

http://www.openbiosystems.com/RNAi/shRNAmirLibraries/TRIPZlentiviralinducibleshR/

Taiwan
GenDiscovery Biotechnology Inc
Phone: [phone]
Fax: [phone]
email: [e-mail]
http://gendiscovery.com.tw/index.html

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