1.which of the following methods is not used to map transcrips?
(a)S1 mapping (b)RNase protection assay (c)primer extension
(d)run-off transcription (e)nuclear run-on transcription
2.a student just purified a plasmid DNA from a bacterial culture.
After 100 times dilution,the absorbance of the plasmid measured
at 260 nm is 0.1 . What is the concentration of this plasmid sample
before dilution?
(a)10 (b)40 (c)300 (d)400 (e)500 μg/ml
3.which of the following mechanisms is involved in the activation of
trp operon genes ?
(a)anti-initiation (b)anti-termination (c)terminator initiation
(d)transcription activation (e)alternative initiator
(f)anti-attenuation
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