[問題]分生的問題~請高手解答一下

[newlife494 (oh~no~)]

1. Please explain why in an E.coli cell the number of tRNA
   is about the same as the EF-Tu(104-105)?
   why the number of EF-Ts (and EF-G) is about the
   same as the ribosome(104)?
   furthermore,why the lac repressor is about only 10 copies?

2.A human cytokine gene"X" has a size of 20 kbp in genomic
  DNA.This gene contains 23 exons and 22 introns,
 10 AGA (Arg) codons,a nuclear localization signal
  sequence,and 6 Cys.What do you need to consider if
 you are cloning and expressingthe gene in E.coli
 to obtain 1 mg of monomeric and active protein.

3.Please design experiments to analyze and
 distinguish 3 activities possessed in reverse transcriptase?

4.Please describe the methods for purification
  of miRNA from the cells and how to demonstrate
  these so purified miRNA have no other RNA contamination.

以上是慈濟考古題啦~但是我跨考所以有看沒有懂，麻煩高手解答一下!!

--
※ 發信站: 批踢踢實業坊(ptt.cc) 
◆ From: 122.127.35.128
→ newlife494:第一題的 104的4是次方5也是