推 ddhamburger:~哇~~!太子學長太強大啦!我看的是當頭棒喝~茅塞頓開~! 10/15 00:08
→ ddhamburger:發不可收拾啊 ~!@完全是了解ㄌ@@@ 10/15 00:09
推 ddhamburger:哈哈 看到標題我嚇ㄌ一跳 10/15 00:14
硬是亂用標題...
這樣應該比較詳細...
N-terminal---Lys's R group---Arg's R group---C-terminal
------------------------------------------------------------------
initial
charge (+1) +1 +1 (-1)
pKa 9.0 10.5 12.5 2.0 SUM
(net charge)
-----------------------------------------------------------------
pH=2 +1 +1 +1 -0.5 +2.5
pH=9 +0.5 +1 +1 -1 +1.5
pH=10.5 0 +0.5 +1 -1 +0.5
pH=12.5 0 0 +0.5 -1 -0.5
thus, pI=(10.5+12.5)/2=11.5
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