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http://en.wikipedia.org/wiki/Sodium_dodecyl_sulfate
Sodium lauryl sulfate
From Wikipedia, the free encyclopedia
(Redirected from Sodium dodecyl sulfate)
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SDS-PAGE
Main article: SDS-PAGE
Sodium lauryl sulfate, in science referred to as sodium dodecyl sulfate
(SDS), is commonly used in preparing proteins for electrophoresis in the
SDS-PAGE technique.[2] This compound works by disrupting non-covalent bonds
in the proteins, denaturing them, and causing the molecules to lose their
native shape (conformation). Also, anions of SDS bind to the main peptide
chain at a ratio of one SDS anion for every two amino acid residues.[citation
needed] This effectively imparts a negative charge on the protein that is
proportional to the mass of that protein (about 1.4 g SDS/g protein).
This new negative charge is significantly greater than the original charge of
that protein. The electrostatic repulsion that is created by binding of SDS
causes proteins to unfold into a rod-like shape thereby eliminating
differences in shape as a factor for separation in the gel. Sodium lauryl
sulfate is probably the most researched anionic surfactant compound. Like all
detergent surfactants (including soaps), sodium lauryl sulfate removes oils
from the skin, and can cause skin and eye irritation. The critical micelle
concentration (CMC) in pure water at 25°C is 0.0082 M[3], and the
aggregation number at this concentration is usually considered to be about
62[4]. The micelle ionization fraction (α) is around 0.3 (or 30%).[5]
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3.
^ P. Mukerjee and K. J. Mysels, "Critical Micelle Concentration of Aqueous
Surfactant Systems", NSRDS-NBS 36, US. Government Printing Office,
Washington,.D.C., 197 1.
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