※ 引述《Xclone (結束 開始 懷念NNN)》之銘言：
: ref38 有提到說acidification會分成兩次完成
: 第一次是extracellular -> early endosome(~pH6)
: 第二次是early endosome -> late endosome
: 之後就才是fusion
: 可是還是沒有提到說
: 到底怎麼樣acidify = ="
baf inhibit的是已經形成early endosome的H-vATPase
而第一次的extracellular -> early endosome(~pH6)
cell biology,vol 130,1995
Transport from Late Endosomes to Lysosomes, but
Not Sorting of Integral Membrane Proteins in
Endosomes, Depends on the Vacuolar Proton Pump
看pg 832最後1段那裡的解釋
The V-ATPase may not be the only factor responsible for pH homeostasis
in late endosomes/lysosomes.Lysosomes maintain a mildly acidic pH after
their isolation in the absence of ATP (Ohkuma et al., 1982). This
acidification does not require an active influx of protons, but can be
abolished by cations such as K ÷ and Na ÷ (Moriyama et al., 1992). Like
Baf, amines and proton ionophores also increase the lyso-
somal pH to 6.0--6.5 (Ohkuma and Poole, 1978).
所以已經形成early endosome了
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※ 編輯: TianKongLan 來自: 140.112.226.125 (06/02 11:48)