求救翻譯
To determine whether calcium oxalate formation plays a role
in regulating calcium transport to low transpiring organs such as
seeds, the calcium, oxalate, and calcium oxalate crystal accumulation
profiles were assessed in the different plant organs of M.
truncatula wild-type and cod5. Microscopic examination of wildtype
tissues revealed a spatial distribution pattern of prismatic
calcium oxalate crystal formation in the cells adjacent to the vascular
bundles in stems, leaves, and pods. Such a spatial pattern of
crystal deposition is consistent with calcium oxalate formation as
a mechanism to restrict calcium transport to low transpiring plant
organs such as seeds [discussed in 16]. The cod5 mutant is devoid
of prismatic crystal formation and thus did not show this pattern
of crystal accumulation. If calcium oxalate crystal accumulation in
the cells adjacent to the xylem stream functions in regulating long
distance calcium transport from the roots to the seeds one would
predict that removal of this mechanism would result in seeds with
increased calcium content.
A comparison of the calcium content of wild-type and cod5
seeds revealed a 60% increase in cod5 seed calcium. Although this
increase is significant, a larger increase would have been expected
based on the estimated amount of calcium that would be “remobilized”
through the prevention of prismatic crystal formation. To
determine if the “remobilized” calcium was being partitioned to
the other plant tissues, calcium measurements were conducted on
cod5 roots, stems, leaves, and pods.
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