組織工程作業第一題...

[newbietheone (Here's your sign=.=)]

因為網路硬碟壞了...

所以PO在班板上...

由於專有名詞多...所以不寫中文版(一下英文一下中文版面會很亂)...

如果有興趣可以翻成中文..

1.
  There are three types of microcarriers currently used in the industry :
A.      Percell porous gelatin
B.      Verax weighted microcarriers
C.      Biomat Informatrix porous microcarriers

Processes used to make these microcarriers:

A.      Percell porous gelatin
  These microcarriers were made from an 8% gelatin solution by a double
 emulsion technique: surfactant was controlled by a double emulsion technique:
 surfactant was added to a warm gelatin solution and the solution was
 saturated with droplets of toluene containing additional surfactant.
 These toluene droplets formed a stable emulsion that created internal voids
 in the microcarrier; the pore size was controlled by the vigor of mixing of
 the solution. Addition of excess toluene created droplets of this emulsion
 in the toluene. The gelatin was then chilled, washed with ethanol and dried.
 The gelatin particles were rehydrated in phosphate buffer, crosslinked with
 glutaraldehyde, washed, autoclaved, and re-dried. In the resulting product,
 the voids were interconnected and thus accessible to cell ingrowth.
 (Nilsson et al.)

B.      Verax weighted microcarriers
  The particles are made from dry milled bovine native collagen, ideally from
 tendon, using a freeze-drying process. The collagen is suspended at about
 0.5-1.5%(w/w) in dilute acids, pH 2.0-4.0, further milled in a blender,
 and mixed with the weighting material-titanium or stainless steel particles
 10-40 μm in diameter. This suspension is then formed into droplets of
 the desired size and frozen, usually in liquid nitrogen; the pore size
 is influenced by both pH and freezing rate. Additional control over
 pore size can be achieved by annealing the particles at -10°C to -20°C
 for at least four hours, after the initial freezing in liquid nitrogen.
 The particles are freeze-dried and crosslinked by a combination of
 dry heat and chemical treatment, ideally by carbodiiamide or
 succinimidyl active esters, then washed and sterilized. (Dean et al.)

C.      Biomat Informatrix porous microcarriers
  Basic processing of collagen-glucosaminoglycan materials begins with
 a thoroughly blended suspension of collagen, preferably from limed bovine hide
 in dilute acid at about 0.25-0.50%(w/v). A solution of glucosaminoglycan,
 usually chondroitin 6-sulfate, is slowly added, forming a co-precipitate.
 This suspension is then formed into sheets(wound dressings) or
 droplets(microcarriers) and frozen at a controlled rate to create
 ice crystals that generate the voids can vary from~1μm to ~800μm
 in diameter, with a void volume fraction of > 99%. The material is
 freeze-dried and crosslinked by thorough dehydration, which crosslinks
 both the collagen  and glycosaminoglucan; the collagen can be futher
 crosslinked by either aqueous or vapor-phase glutaraldehyde.


 單力..我計算題給阿斌摟...


--
*我曾經也有過一張沙發，但是那時候我的腿一點也不酸，所以我走了。
等我腿酸時，再回頭，那張沙發已經被別人搬走了..*---德國詩人 呂克特

所以..要充實地過每一天....該衝一個就要衝...
我的無名相簿..有空常來看歐~~^^
http://www.wretch.cc/album/newbietheone

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