高等醫學生物技術學期考"Take home examination"部份
吳老師部份(10分)
Please describe in detail the procedures of the
cloning and expression of ONE of the following genes:
(a) Human growth factor
(b) Human insulin
(c) Human erythropoietin (EPO)
Hint: You need to describe how to prepare the insert DNA, and the
vector, cloning host, protein expression host, etc. for cloning and
protein expression. In addition, please describe all the necessary
procedures for making sure you get what you intended to obtain.
許老師部份(20分)。
出題範圍如下:TCM-derived polysaccharide and protein in biomedical research and application (I) and (II)。
請同學可自行去下載後閱讀。
注意:同學只要任選一篇文章作答即可。
考題是:請寫出本文章的”漏洞”,例如:實驗設計、結果、討論等的缺失
或可以改進之地方,或可以進一步再研究之實驗,最少指出三點。
Extract of Reishi Polysaccharides Induces Cytokine Expression via Toll-like Receptor 4-modulated Protein Kinase Signaling Pathways.
Reishi Immuno-modulation Protein Induces Interleukin-2 Expression via Protein Kinase-Dependent Signaling Pathways within human T Cells.
以上報告請用A4紙手寫
統一交給班代後交給老師
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