PHYTON 討論會公告
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時間: 18:00-21:00 3-Mar-1999
地點: 圖書館討論室346
主持人: 徐捷
性質: 討論會簡述, 論文--光合作用
題目: Analysis of the chlorophyll biosynthetic system in a
chlorophyll b-less barley mutent
作者: Arkadii B. Rudoi & Rostislav A. Shcherbakov
關鍵字: biosynthetic enzymes, chlorophyll, chlorophyllide, pigment formation,
protochlorophyllide
來源: Photosynthisis Research 58:71-80, 1998
參考閱讀:(a)認何有關chlorophyll biosynthesis的文章(課本上的)
(b)(不勉強)Robert J. Porra, Photochemistry and Photobiology,
1997, 65(3):492-516 (其中的圖....)
附注: (a)第一次總是比較匆忙 所以公告的時間略晚 下次應會改進
(b)摘要來不及翻譯 就照抄了....
(c)以後的公告格式大概就是這樣....
(d)我們大約在17:50於圖書館三樓集合
(e)未竟事宜以後再公布
摘要: The chlorophyll b-less barley (Hordeum vulgare L.) mutant
chlorina 2807 allelic to the well-known barle mutant chlorina
f2 was studied. 5-Aminolevulinic acid at saturating concentration
(40 mM) was introduced into postetiolated leaves of the mutant
and its wild type, and the protochlorophylide accumulation in
the dark was measured. It was found that the activity of the
enzyme system transforming 5-aminolevulinic acid into
protochlorophyllide was the same in both types of plants. The
activity of esterifying enzymes that catalyze attachment of
phytol to chlorophyllide was analyzed by in filtration of exogenous
chlorophyllides a and b into etiolated leaves. The reaction was
shown to have close rates in the mutant and wild-type plants. In
very early stages of greening of etiolated leaves,when the
apoproteins of the light-harvesting complexes are not yet formed,
appearance of chlorophyll b were conversion in any stage of
greening. On the other hand, in the mutant as well as in the wild
type an active reverse conversion of chlorophyll b into chlorophyll a
was possible. It is concluded that (a) in the mutant chlorina 2807
the ability of the biosynthe to transform 5-aminolevulinic acid to
chlorophyll a is fully preserved, (b) in the mutant the enzymes
converting chlorophyll a into chlorophyll b are most likely absent
or damaged, (c) the conversion of chlorophyll a into chlorophyll b
and the reverse conversion of chlorophyll b into chlorophyll a
are performed by different enzymes.
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