About enzyme work in extreme pH environment

Will enzyme denature in extreme pH value environment?



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發信人: [e-mail] (False Memories), 看板: Biology
標  題: Re: About enzyme work in extreme pH environment
發信站: 台大計中椰林風情站 (Sun Jan  3 02:47:58 1999)
轉信站: Ptt!news.ntu!Palmarama

> Will enzyme denature in extreme pH value environment?

Yeah, many proteins unfold at pH values less than 5 and greater
than 10.  (1)It usually occurs because the folded protein has
groups buried in nonionized form that can ionize after unfolding.
(for example, His->unfold at extreme acid pH, Tyr->unfold at
extreme alkaline pH). (2)Extreme pH disrupts salt bridges thus
reduce the stability of the protein.