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念papper 不知道這個語詞是什麼意思 subtraction library The cDNA of the IPO gene was isolated from the subtraction library of sweet potato, and its expression was induced by the application of mechanical wounding and methyl jasmonate (MeJA) 不知道哪裡可以查一些術語 ..程度不好 光搜尋也找不到 可以代為解釋嗎 -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 61.228.190.237 > -------------------------------------------------------------------------- < 作者: Lipaty (小藍大復活!!!!) 看板: Biotech 標題: Re: [問題] subtraction library是什麼 時間: Wed May 18 23:00:59 2005 意思是說IPO這個gene平常表現量低(或是根本不表現) 利用 mechanical wounding and methyl jasmonate來induce 馬鈴薯中IPO的表現之後 用subtraction的方法: (cDNA of induced IPO) - (cDNA of normal) 相減之後,IPO的cDNA會被enriched,變的較原先容易純化。 ※ 引述《DragonFlight (數物化生經世濟用)》之銘言: : 念papper 不知道這個語詞是什麼意思 subtraction library : The cDNA of the IPO gene was isolated from the subtraction : library of sweet potato, and its expression was induced : by the application of mechanical wounding and methyl jasmonate : (MeJA) : 不知道哪裡可以查一些術語 : ..程度不好 光搜尋也找不到 : 可以代為解釋嗎 -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 218.167.44.186
DragonFlight:感謝指點 61.228.190.237 05/18
DragonFlight:IPO是蕃薯的防禦蟲害基因 61.228.190.237 05/18
DragonFlight:不知道是如何相減..hybridization? 61.228.190.237 05/18
aggaci:他的原理很像是microarray..蠻像的!140.114.100.165 05/19
scorpiuswu:http://www.clontech.com/clontech/techinfo/140.122.152.197 05/19
scorpiuswu:manuals/PDF/PT3656-1.pdf140.122.152.197 05/19
scorpiuswu:subtraction library140.122.152.197 05/19
DragonFlight:好方便的網站^^ 61.228.185.241 05/21