Re: [看板活動] 你在看哪篇paper? 10/10--10/25

[niolas (要如何能值得?)]

大四準備專題中,有興趣的就提提問吧!順便可以當做準備 ^^

題目:
Enzymes of the SUMO Modification Pathway Localize to Filaments
of the Nuclear Pore Complex
(SUMO修飾酵素其路徑位於細胞核孔複合物的絲狀體上)

出處: Mol. Cell. Biol. 2002 22: 6498-6508 (2002)

【英文摘要】
SUMOs are small ubiquitin-related polypeptides that are reversibly
conjugated to many nuclear proteins. Although the number of identified
substrates has grown rapidly, relatively little is still understood
about when, where, and why most proteins are modified by SUMO. Here,
we demonstrate that enzymes involved in the SUMO modification and
demodification of proteins are components of the nuclear pore complex
(NPC). We show that SENP2, a SUMO protease that is able to demodify
both SUMO-1 and SUMO-2 or SUMO-3 protein conjugates, localizes to the
nucleoplasmic face of the NPC. The unique amino-terminal domain of
SENP2 interacts with the FG repeat domain of Nup153, indicating that
SENP2 associates with the nucleoplasmic basket of the NPC. We also
investigated the localization of the SUMO conjugating enzyme, Ubc9.
Using immunogold labeling of isolated nuclear envelopes, we found that
Ubc9 localizes to both the cytoplasmic and the nucleoplasmic filaments
of the NPC. In vitro binding studies revealed that Ubc9 and SUMO-1-modified
RanGAP1 bind synergistically to form a trimeric complex with a component
of the cytoplasmic filaments of the NPC, Nup358. Our results indicate
that both SUMO modification and demodification of proteins may occur
at the NPC and suggest a connection between the SUMO modification pathway
and nucleocytoplasmic transport.

【中文摘要】
SUMOs為一種和ubiquitin相關的小分子量多?，可逆的和許多核蛋白結合。雖然許
多基質已經被發現，但很多被SUMO修飾的蛋白質對其時間、位置與原因瞭解不多。
在這我們指出牽涉SUMO修飾與去修飾的酵素為細胞核孔複合物(NPC)的成分。我們
也指出一種可對SUMO-1、SUMO-2、SUMO-3去修飾的蛋白?SENP2位於細胞核孔複合體的
核質面。由於SENP2特殊的N端可和Nup153的FG重複片段反應，故推測SENP2和核質
複合體的籃狀核質相關。我們也測試了SUMO結合酵素Ubc9的位置，利用分離的核膜
做免疫金離子標定，發現Ubc9位於細胞質和核孔複合物的核質絲狀體上。並在試管
研究中發現Ubc9在細胞質絲狀體Nup358存在下會加強和被SUMO-1修飾的RanGAP1結
合。我們的結果指出SUMO的修飾與去修飾可能發生在細胞核孔複合物上，而且SUMO
修飾路徑可能和細胞核質運輸相關。



這樣會不會有點多餘? 有興趣的人可看看review的文章~

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