1.Acid phosphatase is glycoprotein. Cytochemical location of this
enzyme could be approached by traditional enzyme cytochemical methods
and immunocytochemical method. If you are a professor, you are going to
submit a proposal to financial-support organization. How cam you do in
systematic approaches?
2.Cryobiology is a recently well-developed integrated field in biological
and physical sciences. Would you please mention, principle, method,
application and limitations of different cytotechniques, e. q..
cryopreservation of sperm and/or plant calli, freeze-drying,
freeze-substitution, freeze-fracture, freeze-etching and cryoSEM.
3.In the lecture of Botanical Electron Microscopy, we have discussed
a lot about three dimensional images of macromolecule and tissue. Could
you correlate their instrumentation, method and application.
4.gene expression of eukaryotic cells are reflected in forms of RNA(hnRNA)
whose molecular weight is much higher than that of mRNA.
biochemical study of hnRNA slicing is processed by the participation
of small nuclear RNA. Dr. Chow and her adviser approached RNA splicing
with electron microscopic technique and cell fractionation. Could you
figure out a general scheme of their study.
5.Dr. Monday's project proposal on status of Oil Dropletes in Dry Seed
of Zinnia elegans. Now, you are a member of panel reviewer and your
should insist what are the minimal requisite of complementary studies.
6.Point out the importance, application and data interpretation of the
following techniques, such as negative staining, shadowing, and
immunolabelling, etc.,for Molecular and Cell Biology.
7.artifacts and troubles are often found in electron microscope and
the processing of thin section. Could you figure out them in detail
and present the criteria and/or troubleshooting to correct them.
8.compose a solid and meaning paragraph containing the following
terns and techniques without missing any one:
1. Molecular structure
2. TEM
3. SEM
4. Cyrotechniques
5. NMR
6. X-ray diffraction
7. STM
8. Electron diffraction
9. AFM
10. Ultrastructure
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