第一次考試
1. There are three classes of Saccharomyces cerevisiae expression
  vectors. _______ and ________ vectors are used to express foreign
  proteins, while _________ vectors mimics a chromosome since it
  has ________, _______, and ________, is designed to clone a large
  segment of DNA(>100kb)
2. A technique used to introduce free DNA into bacteria, yeast, animal and
   even plant cells.
3. A technique( method ) used to produce defined point mutation in a
   cloned gene.
4. A generalized eukaryotic expression vector is showed below(fig.7.1),
   please use one sentence to explain each term and its function.
   a) p  b) cs   c) t  d) ESM  e) oriE   f) Ampr   g) orieuk
5. What is bacmid ? How is it used ?
6. A vector (system) to express recombinant protein in insect cells.
問答
The advantages and disadvantage of using E. coli , yeast , and mammalian
cells as protein expression system. Which system you would suggest to
use ? why?


期中考
1. 寫出一個reporter gene
2. 寫出2種常用的plant transformation 方法
3. 寫出植物基因工程上常用的promoter
4. 寫出可將外來DNA送到葉綠體的方法
5. 寫出一個可用來做為產生抗植物病原細菌transgenic plant之基因

1. 簡述如何利用plant transformation 的方法來分離一個植物的promoter
2. 如何增強transgene(轉基因) 在植物細胞內的表現
3. 檢述2種可使轉基因蛋白在葉綠體中表現之方法
4. 舉例說明如何產生marker-free 的轉基因植物
5. 簡述最少3種策略可用來產生對昆蟲有抗性之植物
6. 簡述最少3種策略可用來產生對殺草劑有抗性之植物
7. 簡述最少2種策略可用來產生對多種植物病毒有抗性(multiple virus resistance)
   之植物
8. 何謂口服疫苗(edible vaccine)? 簡述如何利用植物產生口服疫苗

一. 請以下列的生合成 pathway
(1) 請舉例說明2個plant transformation 的策略可用來增加B產物的量
(2) 請舉例說明3個plant transformation 的策略可用來降低B產物的量
                    precursor
             enzyme 2   ↓enzyme 1
         C     ←       A
                        ↓enzyme 3
                        B
二. (1)何謂binary vector
    (2)請畫一個binary vector並標示各重要部位以及說明其功能
三. 試述植物基因工程的可能應用


期末考
一. 解釋名詞
a) transgenesis   b) lipofection   c) bacteria fertilizers   d) diazotrophic
e) genetic complementation   f) nif A   g) bioremediation   h) Super bug
I) mesophiles      j) glucose isomerase    k) endoglucanase   l) β-glucosidase
m) single-cell protein(SCP)    n) pesudobactin       o) Agrocin 84
p) positive-negative selection

二. Cloning is the state-of-art technique scientists have cloned sheep,
    cows, and monkey from somatic cells of these animals.
   a) Draw a scheme to show the basic principle of this technique.
   b) Describe the potential problems (scientifically not ethically) of
      this technique.
三. What are the essential requirement for patenting an invention.
四. a) Draw a scheme to show the basic principle of creating transgenic mice?
    b) How gene knockout mice are established(generated).
五. Discuss how transgenesis could be used to improve organ transplantation.
六. a) Outline a scheme for isolation the cluster of no dulation genes
    from Rhizobium japonicum  that can fix nitrogen and from a symbiotic
    relationship with soybean roots.
    b) How the rhizobial host specificity is determined.
六. What are siderphores? How could genetic manipulation of siderophore
    gene enable bacteriato enhance plant growth.
七. Delineate the role of α-amylase and glucoamylase in the industrial
    production of alcohol. How might genetic manipulation of the genes
    encoding these enzymes be used to improve this process.
八. See page345 Q.7