課程名稱︰生物化學概論
課程性質︰選修
課程教師︰丁一倪
開課系所︰化工係
考試時間︰94.4.21
試題 :1.how do gram-negative bacteria differ structurally from gram-positive
bacteria:
1)they have flagella 2)they have an outer membrane
3)they are spherical 3)they contain peptidoglycan
2.下列有關Mitochondria敘述何者正確
1)have a single lipid bilayer
2)are reponsible for photosynthesis
3)synthesize mannose-6-phosphate
4)contain DNA
3.which of folling is not correct
1)mitochondria contain pyruvate dehydrogenase
2)mitochondria contain the enzymes catalyzing fatty acid oxidation
3)mitochondria is the site of eukaryotic
4)mitochondria contain the citric acid cycle enzymes
4.the possible subcellular location for assembling the carbonhydrate
moiety of a glycoprotein is:
1)cytosol
2)smooth endoplasmic reticulum
3)golgi apparatus
4)plasma membrane
5.which is a aldohexoses
1)erythrose
2)fructose
3)galactose
4)ribose
6.the conversion of glucose 6-phosphate to fructose 6-phosphate is
the isomerization of
1)a ketose to an aldose
2)an altrose to an aldose
3)an aldose to a ketose
4)a ketose to an a altrose
5)none of the above
7.which of the following monosaccharide is an important constituent
of nucleic acids and coenzymes?
1)D-glucose 2)D-ribose
3)glycogen 4)D-ribulose
8.lactose is composed of
1)mannose and galactose
2)fructose and glucose
3)mannose and glucose
4)glucose and galactose
5)glucose and gulose
9.the major from of metabolic energy storge in human is
1)triacylglycerols
2)cholesterol
3)fatty acid
4)protein
5)carbonhydrates
10.下列何者是不飽合脂肪酸
1)myristic acid 2)palmitic acid
3)linoleic acid 4)lauric acid
11.下列何者是磷脂的組成成份之一
1)glucose 2)lysine
3)choline 4)folic acid
12.in nucleoside sugar is linked to
1)nitrogen 2)carbone
3)phosphate 4)oxygen
13.the process by which the sequence of bases is passed from DNA
to RNA is called
1)polymerase chain reation 2)translation
3)transcription 4)replication
14.人類mRNA 64 codons 中 除methionine外 下列何種amino acid其codons
只有一個
1)Arg 2)Leu
3)Trp 4)Pro
15.下列何者為蛋白質合成之終止密碼
1)UAA 2)UCC
3)UGG 4)UUU
16.How many surrounding water molecules can from hydrogen bond with
one water molecule
1)2 2)3 3)4 4)5
17.Polor moleculesdiffer from nonpolar molecules in that polar
molecules
1)are soluble in water
2)are strongly positive or negative
3)contain hydrogen bonds while nonpolar molecules contain
covalent bonds
4)usually contain only carbon and hydrogen
18.which of the following molecule can be chracterized as an amphipathic
molecule
1)glucose 2)nucleic acids
3)fatty acids 4)amino acids
19.下列何者為共價鍵
1)ionic bond 2)hydrogen bond
3)disulfide bond 4)hydrophobic bond
20.which of the following statement is correct
1)nucleotide bond is an ester bond
2)nucleotide bond is an anhydride bond
3)peptide bond isan ester bond
4)peptide bond is an anhydride bond
21.which one of the following amino acids would be considered nost polar
1)proline 2)phenylalaine
3)serine 3)valine
22.lysine是一種
1)acidic amino acid 2)neutral amino acid
3)basic amino acid 4)aromatic amino acid
23.the pI of an amino acid is 5.97 its pK1=2.34 what would be the
pK2?
1)10.6 2)8.6 3)none of the above
4)9.6 5)7.6
24.Glutathione 含有那種anino acid
1)alanine 2)serine
3)tyrosine 4)methionine
5)cysteine 6)threonine
25.蛋白質對波長280nm 之光有吸收作用 其最重要之化學結構為
1)peptide bond
2)phenylalanine之苯環
3)tryptophane之indole ring
4)cystine之 SH group
26.由許多個protein subunits組成的立體結構為
1)primary structure
2)secondary structure
3)tertiary structure
4)quaternany structure
27.which of the following sets of three amino acids aare probably
clustered within a protein?
1)Asn,Gly.Lys 2)Tyr,Ser,Lys
3)Phe,Val,Ile 4)Met,Asp,His
28.glubolar protein are characterized by
1)glysine at every third resine
2)large amounts of hydroxyproline
3)many polar and charged amino acids on the outside of the molecule
4)high concentrations of hydrophobic amino acids on the outside of
the molecule
29.which molecule is composed mainly the coiled coil structure
1)β-keratin
2)collagen
3)hemoglobin
4)silk fibroin
5)carboxypeptidase A
30.WHICH ONE OF the following amino acids in a protein molecule
cannot be nodified by a protein kinase
1)Tyr 2)Trp
3)Thr 3)Ser
31.蛋白質之醣化作用可發生在下列那個安基酸上?
1)Asp or Glu
2)Cys or Met
3)Asn,Ser or Thr
4)Gly,Ala or Asp
5)Trp.Asp or Cys
32.please point out which one of the following amino acids is
covalently linked to glycan moiety of a glycoprotein molecule
1)Trp 2)Gln
3)Arg 4)Thr
33.In N-linked oligosaccharides,GlcNAc is invariably β-linked
to the
1)Ser 2)Asn
3)Thr 4)X
residue in the sequence Asn-X-Ser and Asn-X-Thr where X is any
amino acid expect Pro or Asp
34.下列序述何者不正確
1)collagen中最多的amino acid 是Pro
2)Cystine經dithiothreitol反應後可生成cysteine
3)蛋白質之β-turn 結構是由四個amino acids 所構成的
4)BPG可穩定去氧血紅素
5)2,3-BPG DECREASES the affinity of hemoglobin for oxygen in
red blood cells
35.the term"proteome" has been used to describe:
1)the tertiary structure of a protein
2)the structure of protein-synthesizing ribosome
3)regularities in protein structures
4)the complement of protein encoded by an organism's DNA
5)regions(domains) within proteins
36.which amino acid caanot be determined on a regular amino acid using
6M HCl?
1)valine 2)leucine
3)proine 4)serine 5)asparagine
37.which one of the following amino acid found in proteins that can
be converted by treatment with strong base into another amino
acid found in protein?
1)asparagine 2)leucine
3)arginine 4)serinre
38.which one of the following descriptions is not correct
1)Ninhydrin can rected with theα-amino group of amino acids
2)chymotrypsin can cleavaged of peptide bonds on the carboxy
side of the aromatic amino acids
3)FDNB can identification the COOH-terminal residue of aprotein
4)X-ray diffraction can determine the tertiary structure
of a protein
5)ISOELECTRIC FOCUSING CAN DETERMINED the pI of a protein
39.下列敘述何者不正確
1)hydrazinolysis可用於分析蛋白質C-端
2)V8 protease 可把蛋白質中Asp及Glu residues的peptide bond打斷
3)蛋白質進行salting out時,常用之鹽類為ammonium sulfate
4)利用Coomassie Blue 進行蛋白質染色時,此時主要結合作用之amino acid
為鹼性amino acid之 Arg 及芳香族amio acid
5)HPLC可用於決定蛋白質的純度
6)Mercaptoethanol is a reducing reagent
40.有一段peptide(A-T-Y-M-C-G-L-K-M-W-S-P-R-Y)經過溴化氰處理後,
可得幾個PEPTIDE片段?
1)2 2)3 3)4 4)5
41.TO SEQUENCE AN ENTIRE POLYPEPTIDE, the Edman process uses
the phenylistothiocyanate to
1)degrade the carboxy1 group of aspartate and glutamate
2)degrade the amine side chain of lysine and arginine
3)degrade the entire peptide
4)remove theamino-terminal residue
5)remove the carboxy1-terminal residue
42.which one of following method is a protein seperation
procedure based on molecular size
1)isoelectric focusing
2)solvent fractionation
3)molecular-exclusion chromatography
4)ion-exchange chromatography
43.the protein with the property that the binding of a ligand to one
side effects the binding properties of another site on the same
protein is called
1)homotropic protein
2)heteratropic protein
3)recepter protein
4)transmembrane protein
5)allosteric protein
44.enzymes are biological catalysts that enhance the rate of a
reaction by:
1)increasing the amount of freeenergy released
2)decreasing the amount of freeenergy released
3)increasing the energy of the transition state
4)decreasing the activation energy
5)increasing the activation energy
45.which of following description is correct
1)Vmax may approximate a binding constant for the enzyme-substrate
complex
2)Km has the dimensions of molar concentration
3)classic competitive inhibitors lower Vmax but do not affect Km
4)the substrate concentration that produces half-maximal velocity
termed the Vmax
46.下列方程式:1/V=Km/Vmax(1+[I]/Ki)*1/[S]+1/Vmax(1+[I]/Ki)代表:
1)competitive inhibition
2)non-competitive inhibition
3)un-competitive inhibition
4)irreversible inhibition
47.the lower value of Michaelis-Menten constant(Km)for an enzyme means:
1)the affinity between enzyme and substrate is weaker
2)the affinity between enzyme and substrate is stronger
3)its maximal reaction velocity is larger
4)its maximal reaction velocity is smaller
48.最好的基質(substrate)是下列代表的那個值要高
1)Km/Vmax 2)1/Vmax
3)Vmax/Km 4)1/Km
49.A nocompetitive inhibitor will not change
1)Km 2)Vmax 3)Kcat
4)Ki 5)Kcat
50.regulation ofbinding-site(outside enzyme's catalytic site)by
protein phosphorlation is
1)allosteric modulation 2)non-covalent modulation
3)irreversible 4)all of above
51.in any binding system,a sigmoid curve is diagnostic of a
1)competitive 2)inhibitive
3)saturrated 4)cooperative
5)noncompetitive
52.although graphical methods are available for accurate determination
of theVmax and Km od an enzyme catalyzed reaction sometimes these
quantities can be quickly estimated by inspecting values of V
at increasing[S] estimate the Vmax of the enzyme catalyzed reaction
for which the following data were obtained
-------------------------------------------------
[S](M) V(μM/min)
-------------------------------------------------
2.5*10^-6 28
4.0*10^-6 40
1*10^-5 70
2*10^-5 95
4*10^-5 112
1*10^-4 128
2*10^-3 139
1*10^-2 140
---------------------------------------------------
1)~30μM/min
2)~70μM/min
3)~100μM/min
4)~125μM/min
5)~140μM/min
6)2.5*10^-6
7)4.0*10^-6
8)1*10^-5
9)2*10^-5
10)4*10^-5
11)1*10^-4
12)2*10^-3
13)1*10^-2
53.please estinate the Km of the enzyme catalyzed reaction for which
the data were obtained in question 52
1)~30μM/min
2)~70μM/min
3)~100μM/min
4)~125μM/min
5)~140μM/min
6)2.5*10^-6
7)4.0*10^-6
8)1*10^-5
9)2*10^-5
10)4*10^-5
11)1*10^-4
12)2*10^-3
13)1*10^-2
54.which type of enzyme catalyzes the reaction?
R-C-O￣+ATP+NH4＋ ===> R-C-NH2+ADP+Pi
∥ ∥
O O
1)isomerase
2)lyase
3)ligase
4)oxidoreductase
5)phosphorylase
55.摧化下列反應之酵素屬何類(class)之酵素:
ATP+D-glucose <---->ADP+D-glucose 6-phosphate
hexokinase
1)oxide-reductases
2)transferases
3)hydrolases
4)isomerases
56.which of the following enzyme convert hydrogen peroxide into water?
1)suoeroxide dismutase 2)catalase
3)oxgenase 4)peroxidase
57.血液中之glutamate-oxaloacetate transferase
1)需要生物素(biotin)作為輔酵素(coenzyme)
2)於肝損壞時,其活性不會上升
3)在紅血球中為ATP來源
4)在心肌梗塞(myocardial infraction)後,活性會增加
58.the major metabolic reactions of amino acids require the coenzyme
1)biotin
2)thiamine pyrophosphate
3)cyanocobalamin
4)retinol phosphate
5)pyridoxal phosphate
59.Folate 含有那種amino acid
1)glysine 2)glutamate
3)tyrosine 4)methionine
5)tryptophan 6)threonine
60.tetrahydrofolates,THF,are derived from the vitamin folic acid,THF
is a
1)six-carbon carries 2)two-carbon carries
3)five-carbon carries 4)one-carbon carries
5)four-carbon carries
61.which of the following vitamins can modify prothrombin and
other proteins involved in the blood clotting process:
1)vitamin A 2)vitamin K
3)vitamin D 4)vitamin C
5)vitamin E
62.which of the following vitamin is soluble in water?
1)vitamin A 2)vitamin D
3)vitamin C 4)vitamin E
63.how many enzymatic steps are involved in converting glucose to
pyruvate:
1)1 2)6 3)9 4)10 5)12
64.which of the following is not a product of the citric acid cycle:
1)CO2 2)Acetyl CoA
3)ATP 4)FADH2
5)NADH
65.WHAT YIELD OF ATP can be expected from complete oxidation of
Fructose 1,6-bisphosphate in skeletal muscle and the brain
1)28, 2)30, 3)32, 4)34, 5)35 (依新法計算)
二'多選題 (每題一分共25分)
A:(1)對 B:(2)對 C:(3)對 D:(1)(2)對 E:(1)(3)對
F:(2)(3)對 G:(1)(2)(3)對 O:都不對
1.下列敘述何者不正確?
1)OXIDATIVE PHOTOPHORYLATION 發生於粒線體膜上
2)photophosphorylation 發生於葉綠體thylakoid membrane 上
3)蛋白質生合成發生在核糖體上
2.which of following buffering substances are used in maittaining
the hemeostasis of physiological pH condition
1)phosphate buffer
2)hemoglobin solution in the blood
3)cellular amino acids and protein
3.which one of the following description is correct?
1)the major form of glucose in solution is β-D-glucopyranose.
2)one of the structural elements of RNA is β-D-ribose
3)a pyrimidine consists two conjugated rings.
4.v下列敘述何者正確:
1)DNA double helix is stabilized by U-A and C-G hydrogen bonding
2)a major component of RNA but not of DNA is thymine
3)Histone are frequently associated with prokaryotic DNA
5.下列敘述何者正確:
1)Alanine:an aromatic amino acid
2)Proline:the amino acid without a free amino-terminal group
3)Avidin:a protein which is found in egg white and can strongly
bind to biotin
6.下列amino acids 何者帶有branched hydrocarbon chain
1)valine 2)leucine 3)isoleucine
7.下列amino acids何者含有兩個chiral center:
1)cysteine 2)proline 3)threonine
8.下列amino acids何者為極佳amino acid?
1)leucine 2)serine 3)proline
9.下列amino acids何者人類細胞可合成?
1)cysteine 2)methionine 3)proline
10.下列amino acids何者可被iodination
1)serine 2)threonine 3)tyrosine.
11.which of the following amino acids function as "neurtransmiter"
in vivo?
1)glysine 2)glutamic acid 3)phenylalanine
12.which of the following is(are) correct
1)water is simultaneously a hydrogenbond donor and acceptor
2)in an amino acid the α-carbon is bonded to an amino group
and a carboxy group
3)aspartame,L-Asp-Lmethyl ester , is a sweetener (a sugar substitute)
13.which of the following is(are) true?
1)mercaptoethanol is a reducing reagent
2)α-helex,β-sheetandβ-turn are major types of secondary
structures in proteins
3)the presence of α-helex in an unknow protein can be detected
by X-ray diffraction and circular dichroism
14.α -helices are stabilized by which of the force?
1)van der waals force
2)hydrogen bonding between the R groups
3)hydrogen bonds between the CO and the NH units in the peptide
backbone
15.the conformation of a protein (1) is its three-dimensional structure
consists of secondary and tertiary structure of protein
(2)determines the function of the protein
(3)determines the solubility of the protein
17.which one of the following informations cannot be determined or
deduced from the amino acid analyzer?
1)amino acid sequence
2)molecular weight
3)protein comformation
18.which of th following is (are) true?
1)α-helex turn of peptide ond includes 3.2 amino acid residus
2)cyanogen bromide cleaves peptide bonds whose carbonyl function
3)hemoglobin contains Mg ion
19.a major difficulty in extracting recepter proteins from membranes
is that
1)it is difficult to remove the proteins from the membranes without
denaturation
2)there may be very few molecules of the protein on a cell and
the recepter proteins tend to have high molecular weights
3)they always covalently bonded to the membrane lipids
20.which of the following descriptions is correct?
1)regulatory enzymes are usually oligomers
2)pyruvate decoaboxylase is anexample of ligase
3)reactive and nonionizable amino acidresidues in the active
site of an enzyme from its catrlytic center
21.which one of the following descriptions is correct
1)noncompetitive inhibitors bind both E and ES so that EI
AND ESI complexs can be formed
2)allosteric modulators bind to a site other than active site
of theenzyme
3)allosteric enzymes are regulated by noncovalent binding of
modulators
22.which of the following are the ways by which enzymes regulate
their activities?
1)protein phosphorylation
2)the feedback inhibition
3)allosteric effect
23.which of the following description is not correct
1)glucose enters into the glycolytic pathway by phosphorylation
to glucose 6-phosphate,accomplished by the enzyme hexokinase
2)ATP is not a product of the citric acid cycle
3)pyruvate is oxidized to acetyl-CoA by an enzyme know as
pyruvate kinase
24.在citric acid cycle 反應過程中,下列酵素所催化生成的反應,何者有
NADH產生:
1)malate dehydrogenase
2)isocitrate dehydrogense
3)α-ketoglutarate dehydrogenase
25.在citric acid cycle 反應過程中,下列酵素所催化生成的反應,何者有
FADH2產生:
1)malate dehydrogenase
2)succinate dehydrogenase
3)aconitase
三.計算題(共10分)
1.肌肉抽取溶液中的lactate dehydrogenase 經硫酸銨(20~40%)之純化,
得下列數據:
分離步驟 體積 蛋白質 活性
(ml) (mg/ml) (μmole/min/10μl)
粗抽取液 50 32 0.14
硫酸銨(20~40%)分離 10 50 0.65
試求此純化步驟之回收率(recovery)及純化倍數(purification fold)(4%)
2.試求Glutathione 之pI.(2%)
3.what is the pH of the solution by mixing one part of 6mM Na2HPO4
and two parts of 2mM NaH2PO4?(three pKa of H3PO4 are 2.14 ,6.86
and 12.4,respectively ,log2=0.301,log3=o.477).(4%)
βα
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