課程名稱︰生物化學概論
課程性質︰選修
課程教師︰丁一倪
開課系所︰化工系
考試時間︰94,6,23
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1.Maintaining restriction endonuclease resistence after DNA
replication is by
A.methylation C.acetylation
B.phosphorylation D.ethylation
2.What nuclease is reponsile for proofreading duing DNA replication?
A.3'→5' exonuclease C.3'→5' endonuclease
B.5'→3' exonuclease D.5'→3' endonuclease
3.When bacterial DNA replication introduces a mismatch in a double-
stranded DNA, the methyl-directed repair system:
A.cannot distinguish the template strand from the newly replicated
strand
B.changes both the template strand and the newly replicated strand
C.corrects the DNA strand that is methylated
D.corrects the mismatch bychanging the newly replicated strand
E.corrects the mismatch bychanging the replicated strand
4.The zinc finger motif is present in a number of DNA binding proteins.
The amino acid residues of a zinc finger protein coordinated to a
zinc ion are
A.His,Arg B.Arg,Cys C.His,Cys D.His,Arg,Cys
5.下列那一種與RNA 生合成無關？
A.Enhancer C.Cap binding protein
B.Promoter D.Transcription faction
6.The sigma factor (σ) of prokaryotic RNA polymers is part of the
core enzyme.which one of the following statement is not ture for
sigma factor?
A.It binds transiently to the core and directs the enzyme to specific
binding sites on the DNA.
B.It is released from the core enzyme after the first phosphodiester
bond has been formed.
C.It must be present for transcription to occur.
D.It specifically recognizes the promoter sites.
7.Which of the following statements about eukaryotic RNA synthesis is
correct ?
A.Polymerse I is required for mRNA synthesis.
B.α-amanitin can block polymerase I activity.
C.All three polymerase need the same cofactors for transcription.
D.Termination of transcription requires the protein ρ factor.
E.the primary mRNA transcript is inactive.
8.Which one of the following is not a basal transcriptional factor
for eukaryotic cells.
A.TFIIE C.GC box binding protein Sp1
B.TFIIH D.TATA box binding protein (TBP)
9.Which one of the following RNA has the highest percentage of modi-
fied bases ?
A.mRNA B.tRNA C.rRNA D.hnRNA
10.AZT used in the treatment of HIV infection, can interfere with a
protein encoded by HIV genetic material but not by muman genome.
AZT acts in HIV-infected cells by:
A.inhibiting reverse transcriptase.
B.blocking ATP production.
C.inhibiting RNA processing.
D.inhibiting RNA polymerase II,
E.blocking deoxynucleotide synthesis.
11.下列那一個化合物為最佳之ribozyme可進行splicing ?
A.AMP B.CMP C.GMP D.TMP
12.If the sequence of a segment of mRNA is 5'-GCAUUCUUAAAA-3',the DNA
sequence complementary to it is
A.5'-GCATTCTTAAAA-3' C.5'-TTTTAAGAATGC-3'
B.5'-CGTAAGAATTTT-3' D.5'-AAAATTCTTACG-3'
13.Which one of the RNA fragments can from a hairpin?
A.UACGAAGUUCGUA C.CACAAGGUCCGGA
B.UGCGCAGUCUGUG D.CACGGAGCUCCUG
14.有關原核細胞基因之轉錄及轉譯,下列敘述何者為非 ?
A.當基因轉變為mRNA可同時進行轉譯為蛋白質
B.所有的mRNA皆為monocistronic, 只能合成單一蛋白質
C.催化轉錄的酵素為DNA-dependent RNA polymerse
D.其Genetic code和真核生物細胞是一樣的
15.Which od the following statement is correct ?
A.The encoding information os protein on DNA must be continuous.
B.Transcription always starts at AUG codon.
C.Translation always starts at the beginning end of mRNA.
D.No processing reaction for all RNA transcripts.
E.None of the above
16.Degeneracy of the genetic code denotes the existence of:
A.codon consisting of 2 bases
B.multiple codons for a single amino acid
C.a given codon codes for different amino acids
D.codons that include one or more of the "unusual" bases.
17.Which of the following are features of the wobble hypothesis ?
A.A naturally occurring tRNA exists in yeast that can read both
arginine and lysine codons.
B.A tRNA can recogize only one condon.
C.The "wobble" occurs only in the first base of the anticodon.
D.Some tRNAs can recognize codons that specify two different
amino acids, if both are nonpolar.
E.The third base in a codon always forms a normal Watson-Crick base
pair.
18.Which of the following is true about the genetic code ?
A.All codons recognized by a given tRNA encode different amino acids.
B.Several different codons may encode the same amino acid.
C.It is absolutely identical in all living things.
D.The base in the middle position of the tRNA anticodon sometimes
permits "wobble" base pairing with 2 or 3 different codons.
E.The first position of the tRNA anticodon is always adenosine.
19.t-RNA 在蛋白質合成過程, 被稱為 "Adaptor"是因為
A.t-RNA 可根據蛋白質序列合成mRNA
B.t-RNA 可將50S 及30S ribosomal subunits結合在一起
C.t-RNA 可 "READ" mRNA序列, 同時又可攜帶特定的胺基酸
D.t-RNA 可被特定的aminoacyl tRNA synthetase "Recognize",形成charged
t-RNA
20.The t-RNAs have 4 base-paired stem.To which loop or stem amino acids
are charged ?
A.the T loop
B.the D loop
C.anticodon loop
D.the acceptor stem
21.有一t-RNA 之anticodon 為5'-GGU-3',其相對應之mRNA codon最可能是：
A.5'-ACU-3' C.5'-CUA-3'
B.5'-CCA-3' D.5'-UCA-3'
22.Which of the following statements about tRNA molecules is false ?
A.A,C,G and U are the bases present in the molecule.
B.Although composed of a single strand of RNA, each molecule con-
tains several short,double-helical regions.
C.Any given tRNA will accept only one specific amino acid.
D.There is at least one tRNA for each of the 20 amino acids.
E.The amino acid attachment is always to a C nucleotide at the 3'
end of the molecule.
23.In the "activation" of an amino acid for protein synthesis:
A.there is at least one specific activating enzyme and one specific
tRNA for each amino acid.
B.leucine can be attached to ｔＲＮＡphe, by the aminoacyl-tRNA
synthetase specific for leucine.
C.methionine is first formylated, then attached to a specific tRNA.
D.the amino acid is attached to the 5' end of the tRNA through a
phosphodiester bond.
E.two separate enzymes are required, one to form the amonoacyl
adenylate, the other to attach the amino acid to the tRNA.
24.已知真核細胞合rRNAs:r-28S,r-18S,r-5.8S,r-5S 共四種, 已知其三個形成
一個precursor 其三個為:
A.r-28S,r-18S 與r-5.8S C.r-28S,r-18S 與r-5S
B.r-28S,r-5.8S與r-5S D.r-18S,r-5.8S與r-5S
25.One isolated a full-length mRNA with 1,000 nucleotides which could
encode a protein with a molecular weight of:
A. 30,000 C.120,000
B. 60,000 D.240,000
26.Which is not used in post-translational modification for insulin
synthesis ?
A.nuclear localization
B.disulfide linkage
C.protease cleavage
D.folding
27.Which of the following statements about regulation of the lac operon
is true ?
A.Glucose in the growth medium does not affect the inducibility by
lactose.
B.Glucose in the growth medium increases the inducibility by lactose.
C.Glucose in the growth medium decreases the inducibility by lactose.
D.Its expression is regulated mainly at the level of translation.
E.The lac operon is fully induced whenever lactose is present.
28.IPTG (isopropylthiogalactoside) induction of β-galactosidase
activity is the result of
A.stimulation of Lac repressor function.
B.IPTG binding to the lac operon and inducing transcription.
C.IPTG binding to the lacI gene product and inhibiting its
activity.
29.Frederick Sanger developed the dideoxy method for DNA sequencing.
What is the principle that Sanger based on ?
A.hybridization
B.replication
C.transcription
D.restriction enzyme mapping
E.inhibition of β-galactoside degradation.
30.下列何者不是Cumarin 與lignin 之共同precursor:
A.mevalonic acid
B.cinnamic acid
C.phenylalanine
D.prephenic acid
31.下列何者為flavonoid 與lignin 之共同precursor:
A.phenylalanine
B.cinnamic acid
C.coumaric acid
D.all of the above
32.Flavonoides、indole alkaloids 及aromatic secondary metaboliteds 可經由
下列那個路徑或循環生成：
A.citric acid cycle
B.glyoxylic cycle
C.uronic acid pathway
D.shikimic acid pathway
33.下列何者為theanine之precursor:
A.alanine
B.ethanolamine
C.glutamic acid
D.all of the above
34.下列何者不是由isopentenyl pyrophosphate衍生而來的：
A.steroids
B.carotenoids
C.gibberellin
D.phytol
E.tropine
F.lycopene
35.下列何者為purine alkaloids之precursor:
A.asparagine
B.proline
C.ornithine
D.all of the above
36.下列何者不是Phe、Trp與Tyr 之共同precursor:
A.shikimate
B.chorismate
C.prephenate
D.phenylpyruvate
37.How many trips through β-oxidation would a 16-carbon fatty acid
need to be completely metabolized ?
A. 6 B. 7 C. 8 D. 9
38.Which statement of oxidation of palmitoyl-CoA is correct?
A.involves 8 rounds of β-oxidation.
B.yields 8 ＦＡＤＨ2
C.yields 7 NADH
D.yields 7 acetyl-CoA
39.The enzyme,ATP-citrate lyase,is important in the biosynthesis
of fatty acid.Where is it located ?
A.mitochondria C.lysosome
B.cytosol D.nucleus
40.Choose the INCORRECT statement describing fatty acid synthesis:
A.requires acetyl-CoA as precursor
B.requires NADPH and ATP
C.requires biotin
D.start at carboxyl end
E.β-hydroxylacyl intermediates have the D configuration
F.Acetyl-CoA carboxylase催化Acetyl-CoA → Malonyl-CoA
G.The rate-limiting step in fatty acid synthesis is the reaction
catalyzed by acetyl-CoA carboxylase.
41.Which of these can be synthesized by plants but not by humans ?
A.palmitate
B.pyruvate
C.linoleate
D.stearate
E.phosphatidylcholine
14
42.If malonyl-CoA is synthesized from radioactive ( Ｃ-labeled )
carbon dioxide and unlabeled acetyl-CoA,and the labeled malonate
is then used for fatty acid synthesis, the final product (fatty
acid) will have radioactive carbon in:
A.every C.
B.every odd-numbered C (carboxyl end is C-1)
C.every even-numbered C.
D.only the farthest carbon from C-1
E.no part of the molecule.
5,8,11,14
43.In animal, the Ｃ20:4,△ fatty acid can be synthesized from
9 9,12
A.Ｃ16:1,△ C.Ｃ18:2,△
9 9,12,15
B.Ｃ18:1,△ D.Ｃ18:3,△
44.The more active elongation of fatty acid chains occurs in
A.lysosome C.endoplasmic reticulum
B.nucleus D.mitochondria
45.Leukotriene is converted from arachidonate by which of the following
enzyme ?
A.Phospholipase D.HMG-CoA reductase
B.Lipoxygenase E.Cyclooxygenase
C.Lipid kinase
46.在脂肪細胞中將triacylglycerol 分解成free fatty acid及glycerol
的酵素是
A.Pancreatic lipase C.Hormone sensitive lipase
B.Hepatic lipase D.Lipoprotein lipase
47.An example of a glycerophospholipid that is involved in cell
signaling is
A.arachidonic acid.
B.ceramide.
C.testosterone.
D.phosphatidylinositol.
E.vitamin A (retinol).
48.A compound containing N-acetylneuraminic acid (sialic acid) is:
A.cardiolipin.
B.sphingomyelin.
C.ganglioside GM2.
D.phosphatidylcholine.
E.platelet-activating factor.
49.Which of the following statement is incorrect?
A.Triacylglycerols are not found in the animal membranes.
B.Glycine is not present in the menbrane phospholipids.
C.Phospholipase Ａ2 can hydrolyze phosphatidylcholine.
D.EPA is not sterols.
E.Acetic acid is the precursor for biosynthesis of cholesterol.
F.Cholesterol is not made from isopentenyl pyrophosphate.
50.About cholesterol metabolism, which of the following is NOT CORRECT ?
A.HMG-CoA reductase is the primary control site for cholesterol
biosynthesis.
B.HDL receptor activity governs cholesterol removal from the blood.
C.The rate of esterification of cholesterol is controlled by ACAT.
D.ACAT stands for acyl-CoA:cholesterol acyltransferase.
51.Which of these statements about the regulation of cholesterol
synthesis is not true ?
A.Failure to regulate cholesterol synthesis predisposes humans to
atherosclerosis.
B.High intracellular cholesterol stimulates formation of cholesterol
esters.
C.Cholesterol acquired in the diet has essentially no effect on
the synthesis of cholesterol in the liver.
D.Insulin stimulates HMG-CoA reductase.
E.Some metabolite or derivative of cholesterol inhibits HMG-CoA
reductase.
52.Which of these statements is not true ?
A.3-hydroxy 3-methylglutaryl-CoA is an inhibitor of HMG-CoA reductase.
B.cholesterol is not classified as lipoproteins.
C.LDL is the most dangerous form of cholesterol to cause athero-
sclerosis.
D.LDL contains Apo B-100.
E.Chylomicron remnants can be uptaken by the liver through LDL-recep-
tor or LPR mediated endocytosis.
53.下列敘述何者不正確：
A.Apo CII 因為mutation而失去功能時, 可能造成血液中triacylglycerol 升高。
B.Estradiol可由膽固醇轉化而成。
C.7α-hydroxylase是由cholesterol 合成bile acids過程中的關鍵酵素 ( key
enzyme)
D.Bile acid is inhibitor of cholesterol 7α-hydroxylase.
E.Eicosanoids is not synthesized from isopentenyl pyrophosphate.
54.In amino acid catabolism,the first reaction for many amino acids is
a(n):
A.decarboxylation requiring thiamine pyrophosphate (TPP)
B.reduction requiring pyridoxal phosphate (PLP)
C.transamination requiring pyridoxal phosphate
D.hydroxylation requiring NADPH and Ｏ2
E.oxidative deamination requiring NAD+
55.Which of the following statements about the fixation of atmospheric
nitrogen (Ｎ2) INTO NH3 by living cells is false ?
A.It requires a source of electrons, normally ferredoxin.
B.It requires two key protein components,both containing iron.
C.It requires one ATP per mol of Ｎ2 fixed.
D.It occurs in certain microorganisms,but not in humans.
E.It involves the transfer of 8 electrons per mol of Ｎ2.
56.About amino acid deamination, which of the following is not correct ?
A.Most amino acids are deaminated by transamination.
B.The predominant amino group acceptor is α-ketoaspartate, pro-
ducing aspartate and the new α-keto acid.
C.The enzyme that catalyze transamination are called transaminases.
D.The enzyme that catalyze transamination are called aminotrans-
ferases.
57.The major metabolic reactions of amino acids require the coezyme:
A.thiamine pyrophosphate D.biotin
B.cyanocobalamin E.pyridoxal phosphate
C.retinol phosphate
58.Which of the following statements is false ?
A.Lys is not degraded to pyruvate.
B.Cys is actively involved in biosynthesis of glutathione.
C.In human and other primates, the final product of purine degra-
dation is uric acid.
D.Gout is cause by an excess of uric acid.
E.Asp is directly synthesized from oxaloacetate in citric acid cycle.
59.Which of the following statements is false ?
A.Tetrahydrofolates,THF,are derived from the vitamin folic acid, THF
is a one -carbon carries.
B.α-ketoglutarate is the precursor of proline, ornithine, and argin-
ine.
C.Deficiency in dopamine production in certain areas of the brain
is associated with Parkinson's disease,a degenerative condition
causing "shaking palsy".
D.CTP is formed by amination of UTP.
E.The first nucleotide product in the de novo biosynthestic pathways
of purines is IMP.
60.Which of the following molecules are not involved in the negative
feed-back regulation of producing 5-phosphoribosyl 1-amine during
purine synthesis.
A.XMP D.ATP
B.GDP E.all of the above
C.ADP
61.下列有關尿酸(uric acid) 之敘述, 何者為正確 ?
A.尿酸是thymidine 之分解產物
B.尿酸缺乏是造成痛風之主要原因
C.尿酸可經由uricase 作用, 方能分泌至人類尿液得以排泄
D.Hypoxanthine經xanthine oxidase作用產生尿酸
62.Which of the following compounds not belong to catecholamines
A.norepinephrine C.serotonin
B.epinephrine D.dopamin
63.In biosynthetic pathway, which amino acids group has a common
precursor ?
A.His,Tyr,Gly C.Val,His,Leu
B.Glu,Pro,Leu D.Ser,Cys,Gly
64. 下列敘述何者正確 ?
A.胺基酸之iodination發生在threonine 上。
B.Glu 是人類細胞可以合成的胺基酸。
C.A glucogenic amino acids is one that yieled acetyl-CoA on
degradation.
D.The N1 of purines arises from the amino group of Gly.
E.Birds convert waste nitrogen to the less toxic product urea.
65. 下列敘述何者不正確 ?
A.Folate含有glutamate.
B.Glutamate is an important carrier of ammonia-derived nitrogen.
C.The synthesis of fumarate is a link between the urea cycle and
the citric acid cycle.
D.Epinephrine is formed from norepinephrine by methylation.
E.S-adenosylmethionine is the methyl group donor for various
methyltransferases.
66.For a chemical reaction A + B ←→ C + D, the change of its free
energy △Ｇ = (ＧC + ＧD) - (ＧA + ＧB) and the change of standard
free energy △Ｇ° = (ＧC° + ＧD° ) - (ＧA° + ＧB° ) which of the
following statement is correct ?
A.△Ｇ is a constant at fixed temperature.
B.A + B → C + D is energetically favorable only when △Ｇ° is
negative.
C.A + B → C + D is energetically favorable only when △Ｇ is
negative
D.All of the above.
67.Would you expect the △Ｇ'° for the hydrolysis of a thioester to be
A.large and negative
B.large and positive
C.small and negative
D.small and positive
68.In electron transport, electrons are carried from Complexes I
And II to Complexes III by ?
A.by Cytochrome C
B.by Coenzyme Q
C.by Succinate oxidoreductase
D.by cytochrome C oxidase
E.by Cu(II)
69.In electron transport system, electrons are carried from
A.Complexes III to Complexes IV by NADH.
B.Complexes III to Complexes IV by cytochrome c.
C.Complexes III to Complexes IV by coenzyme Q.
D.Complexes III to Complexes IV by ubiquinone.
70.About ATP synthetase, which is not correct ?
A.ATP synthetase is also known as Complex V.
B.ATP synthetase is also known as sodium-pumping ATP synthase.
C.ATP synthetase is also known as proton-pumping ATP synthase.
D.ATP synthetase is also known as Ｆ１Ｆo ATPase.
E.ATP synthetase is a multisubunit transmembrane protein with
a total molecular mass of 450KD.
71.When hydrogen ions are pumped from the mitochondrial matrix,
across the inner membrane, and into the intermembrane space,
the result is:
A.the formation of ATP
B.the creation of a proton gradient.
C.the restoration of the Na+-K+ balance the membrane.
D.the lowering of pH in the mitochondrial matrix.
72.Which of the following statement is NOT a accurate description
about Calvin cycle.
A.It is a carbon assimilation process.
B.In this process carbon dioxide is reduced to organic sugars.
C.This set of reaction is often called the light reaction.
D.The Calvin cycle is a means of carbon fixatioin.
73.The reactions catalyzed by Rubisco can be summarized schema-
tically as
A.Ｃ6 → 2Ｃ2 + ＣＯ2
B.ＣＯ2 + Ｃ5 → 2Ｃ3
C.3Ｃ5 → Ｃ3 + 2Ｃ6
D.Ｃ6 + 3Ｃ3 → 3Ｃ5
74.Which enzyme fixes ＣＯ2 in mesophyll cell of Ｃ4 plant
A.Rubisco (ribulose-1,5-bisphosphate carboxylase)
B.Acetyl-CoA carboxylase
C.Malic enzyme
D.PEP carboxylase
75.G protein can be activated on binding with
A.GMP
B.GDP
C.Guanine triphosphate
D.GTP
E.all of the above
二、選擇題 (多選, 每題 1分, 共20分)
A:(1)對, B:(2)對, C:(3)對, D:(1)(2)對, E:(1)(3)對, F:(2)(3)對,
G:(1)(2)(3)對, 0:全部不對
1.Which of the following statement about DNA replication is incorrect?
(1) DNA replication occurs at S phase
(2) In DNA replication DNA polymerase Ⅲ is used for polymerization
of leading strand and lagging strand.
(3) The direction of lagging strand DNA synthesis is opposite to
the direction of movement of replication fork.
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