課程名稱︰遺傳學
課程性質︰必修
課程教師︰于宏燦
開課學院：生科院
開課系所︰生科系
考試日期（年月日）︰2013/3/25
考試時限（分鐘）：3hr
是否需發放獎勵金：是
（如未明確表示，則不予發放）
試題 :
此為共同授課
範圍為2~6章-于宏燦老師 20~21章-郭志鴻老師
于老師部分 60%
I. Terminology(40pts)
1. Genetic recombination
2. Norm of reaction
3. Breed true
4. Submetacentric chromosome
5. AFLPs
6. Penetrance
7. Overdominance
8. Genomic imprinting
9. Complementation
10. Maternal effect
11. Barr body
12. Extranuclear inheritance
13. Non-parental type
14. Testcross
15. Genetic linkage map
16. Pseudoautosomal region
17. Heterogametic sex
18. Dosage compensation
19. Genetic markers
20. Reciprocal cross
II. Essay questions(20pts)
1. Below is a metaphase of a cell division
(想像有一個V和三個形狀不同的X在中央板上 姊妹染色體分離)
1.1 Is this mitosis or meiosis?
1.2 If this is meiosis then draw an image of mitotic metaphase of this cell.
If this is mitosis then draw an image of meiotic metaphase of this cell.
1.3 Explain why you have your answers?
2. Some explorers brought back two plants that were discovered in a remote
mysterious valley. In appearance, the two plants look alike and have
bisexual flowers. However, one produces round seeds and the other square
seeds. Acoording to the discoveres, the plants were abundance in the
valley, and they saw much more plants that were round-seed-bearing than
square-seed-bearing. How would you design experiments to study the genetic
mechanism that determines the shape of seed?
郭老師部分(40%)
1.(2pt) The term "genome" refers to ________________.
2.(2pt) _____________ uses the unique banding patterns of a chromosome to
determine the general location of a gene.
3.(4pt) ____________ relies on DNA cloning techniques to determine the
location of genes; the distances are computed in _______________.
4.(2pt) DNA microarrays can be used to monitor gene expression at the level of
____________. (hint: DNA, RNA, or protein?)
5.(6pt) List three possible explanations on why the proteome of an organism
may be larger than is genome.
6.(4pt) If two genes are derived from the same ancestral sequence, they are
called _____________. If these two genes are found in two different
species, they are further defined as _______________.
7.(6pt) Assuming that you are planning to clone a bacterial genome (~5.4Mb)
into a fosmid library (average inser size = ~40kb), how many clones
do you need to achieve an estimated coverage of 6-fold?
8.(6pt) Assuming that you are preparing a grant proposal to sequence the
genome of a fungus. Given the information below, please estimate the
sequencing cost of this project.
a. The genome size is estimated to be ~20 Mb.
b. Using the shotgun sequencing approach, the goal is to provide an
estimated coverage of 8-fold for assembly.
c. The sequencing facility charges ＄100 for each Sanger sequencing
read. The average read length is 800 bp.
9.(8pt) Use a dot matrix to calculate the sequence identity(%) between
"MNTKELWTEV" and "MNTREIWTEV".
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※ 編輯: aki80 來自: 140.112.4.206 (04/08 16:51)