[試題] 2003 生化乙 第一次期中考題

[enisx (Dr板 歡迎您的光臨指導)]

課程名稱︰生物化學乙上
課程性質︰必帶or必選or選修
課程教師︰張震東 蔡蔭和 果伽蘭
開課系所︰生化所
考試時間︰2003.11.3 ch.1-7 (蔡蔭和部分)

試題 :

一. Problems of multiple choice

__ 1.At pH7,anionic phospholipids are (a)PS (b) PA (c) PC (d)PE (e)all the
     above.

__ 2.In phenylketouria patient,abnormal level of the following is accumulated
     in the blood and urine (a)glucose (b)acetyl-coA (c)phenylpyruvate
     (d)phenylalanine (e)acetone

__ 3.Coagulation factors in vertebrate blood may contain a special amino acid
     which is involved in Ca2+ - binding (a)aspartic acid (b)glutamic acid
     (c)Proline (d)gamma-carboxyl glutamic acid (e) histidine

__ 4.A heteropolymer (a)consists of one kind of monomer (b)consists of more
     than one kind of monomer ,and may be linked in any order (c)is a mixture
     of molecules of different sizes (d)can contain more biological
     informations than a homopolymer can (e)probably are produced from more
     than one gene

__ 5.Which reaction(s)_is involved in the ribozyme's self-splicing catalytic
     activity?(a)transesterfication (b)peptide bond hydrolysis (c) amide
     hydrolysis (d)phosphodiester bond hydrolysis (e)double bond cleavage

__ 6.Tris-HCl is an useful buffer for in vitro biochemical reaction and its
     pH buffering range is (a)4.5-6.5 (b) 6.5-7.2 (c)7.2-9.2 (d)9.2-11.2
     (e)3.0-4.8

__ 7.Biological catalysis are (a)proteins exclusively (b)RNA exclusively
     (c) DNA exclusively (d) some proteins and some RNA (e) some proteins and
     lipids

__ 8.A protein composed of 210 amino acid has a molecular weight of about
     (a)20,000 (b)14,000 (c)23,000 (d)28,000 (e)may >24,000 if contains
     glycan

__ 9.The sweet peptide commonly used id (a) D-Asp-D-phe-OCH3
     (b)L-Asp-L-phe-OCH3 (c) L-Asp-D-phe-OCH3 (d)L-Asp-L-phe-OH
     (e)L-Asp-L-Tyr-OCH3

__ 10.Fetal hemoglobin (HbF) has higher affinity for O2 than does maternal
      hemoglobin because (a)the pH of the fetus is lower (b)lower affinity
      of HbF for bisphosphoglycerate;BPG (c) higher affinity of HbF for BPG
      (d)HbF is alpha2-gamma2 (e)hormone may regulate the local level BPG

__ 11.The most important force involved in the stablization secondary
      structure of proteins is (a)electrostatic salt bridge (b)hydrophobic
      interaction (c)hydrogen bondings (d) all the above (e)dipole-dipole
      interaction

__ 12. Result from amino acid analyzer can give us information about the
       proteins (a)A.a composition (b) A.a sequences (c) PI value
       (d)all the above (e) Mass

二.簡答題

1.Briefly explain the double-origin theory of "the origin of life"

2.Please compare the differences in the existence of organelles in prokaryotic
  and eukaryotic cell.

3.What are the roles played by llysomes and peroxisomes,respectively?

4.Name the most important physiological buffer system in cells and in blood.

5.What is Henderson-Hasselbalch equation?

6.Use 3-letter-codes to name two amino acide with basic side chain, and the
  other two with carboxyl side chain.

7.Show the chemical structures of the side chain of Met and Tyr at neutral pH
  .
8.Stetch resonance-structure for the peptide group.

9.Which two uncommon amino acids are present in collagen?

10.Please define the secondary structure of a protein

11.Name the four lipid-soluble vitamins.

12.Calculate the PI of a tripeptide Gly-His-His (note: the 3 pKa of His are
   1.8, 6.0, 9.2 and the 2 pKa of Gly are 2.3, 9.6 )

13.Name two amino acids which are common and essential for the formation of
   "reverse turn"

14.Give the full names of fatty acids or acyl groups which covalently link to
   the side chain of proteins and helpthem to anchor the membrane bilayse

15.Use English to name two families of very important compounds
  (ie.eicosanoids) derived from arachidonate

16.Give an examples of "facilitated-diffusion,and briefly explain

17.explain the fluid mosaic model.

18.A charged sugar is linked to galactose moiety in all gangliosides ,
   what is its English name and another common name?

19.Explain the structural relations of myoglobin and hemoglobin

20.Which metal ions are present in the active site of carboxypeptidase?
   What would happened if a chelating agent (eg EDTA) is added to the
   enzyme reaction?

21.Which resides (including Numbering) in chymotrypsin play the roles of
   nucleophile and general base, respectively?

22.Members of an enzyme superfamily may evolve different substrate specificties
   ,give an example.

23.Would salt concentration in the reaction buffer affect the enzyme kinetics
   of trypsin and chymotrypsin? Please predict how the [NaCl] at 0.01-0.4M
   affect the catalytic rate of each enzyme

24.How are plots of reaction-rate vs [S] may distinguish an allosteric enzyme
   from a non-allosteric enzyme?




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※ 編輯: enisx           來自: 140.112.25.191       (11/27 09:36)
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