課程名稱︰ 分析化學乙下
課程性質︰ 系上必修
課程教師︰ 劉春櫻
開課學院： 理學院
開課系所︰ 化學系
考試日期（年月日）︰ 2008/6/18
考試時限（分鐘）： 140 minutes
是否需發放獎勵金： 是
（如未明確表示，則不予發放）
試題 :
1. Explain the following terms: (24%)
(1) linear flow rate
(2) asymmetry factor
(3) Kovats retention index
(4) molecular sieve
(5) sulfur chemiluminescence detector
(6) supercritical fluid chromatography
2. If selectivity factor, α≒1, optimizing k' and increse N cannot get a
satisfactory separation performance within a reasonable time. How will you
do to improve the selective factor? (8%)
3. Match the terms with their definitions: (10%)
(1) Adsorption chromatography
(2) Partition chromatography
(3) Ion-exchange chromatography
(4) Molecular exclusion chromatography
(5) Affinity chromatography
-(a) Mobile phase ions attracted to stationary phase ions.
-(b) Solute attracted to specific groups attched to stationary phase.
-(c) Solute equilibrates between mobile phase and surface of stationary
phase.
-(d) Solute equilibrates between mobile phase and stationary liquid film.
-(e) Solute penetrates voids in stationary phase. Largest solutes eluted
first.
4. Give the principles of capillary electrophoresis. (10%)
5. Why does the direction of electroosmotic flow changes when a silica
capillary is washed with a cationic surfactant? (5%)
6. How a reversed-phase column can be used as an ion-exchange column for the
separation of a protonated organic base? (6%)
7. Illustarte the principles of ion chromatography for the anion separation of
the mixture of NaNO3 and CaSO4. (10%)
8. How can ion exchangers be used in (1) preconcentration of trace metal ions
(2) preparation of deionized water. (8%)
9. The following analytes in aqueous solution were extracted by dithizone into
CCl4. What information can be given from the plots "percentage extracted
vs. pH"? (8%)
[view Figure 23-4 in *.]
[* Harris, D.C. 2007. Quantitative Chemical Analysis. 7th ed.
W.H. Freeman, USA. p. 505.]
10. Why do bands spread in chromatography? Please explain it in terms of van
Deemter equation. (12%)
11. Describe the following three detection methods for the gas chromatography.
(1) thermal conductivity detector
(2) flame ionization detector
(3) electron capture detector. (15%)
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