課程名稱︰ 分析化學乙下
課程性質︰ 系所必修
課程教師︰ 劉春櫻
開課學院： 理學院
開課系所︰ 化學系
考試日期（年月日）︰ 2008/4/23
考試時限（分鐘）： 120 min
是否需發放獎勵金： 是
（如未明確表示，則不予發放）
試題 :
1. Define the following terms: (24%)
a) stray light b) matrix modifier
c) photodiode array spectrophotometer d) hollow cathode lamp
e) flow injection analysis e) chemiluminescence
2. What are the advantages and disadventages of decreasing monochromator slit
width? (3%)
3. Describe two mechanisms broading the lines to 10^-3 to 10^-2 nm in atomic
spectroscopy. (6%)
4. Explain how the background correction techniques work: (a) beam chopping,
(b) Zeeman (8%)
5. Explain what is meant by spctral, chemical, ionization interference. (9%)
6. How to get the composition of a complex with the method of continuous
variation? (10%)
7. Explain how a laser generates light. List improtant properties of laser
light. (10%)
8. Describe the differences between the following and list any particular
advantages possessed by one over the other: (18%)
a) phototubes and photomultipier tubes
b) conventional and diode-array spectrophotometers
c) fluorescence immunoassay and time-resolved fluorescence immunoassay.
9. (a) What is aptamer? (b) To measure the protein immunoglobulin, an aptamer
is seleted that binds IgE. Describe how "ruthenium complex with a flat
aromatic structure" can be used as "molecular light switch." (10%)
10. Describe the principle for the luminescent determination of intracellular
O2 concentration with 4,7-diphenyl-1,10-phenanthroline Ru(II) complex.
(10%)
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※ 編輯: charmaxim 來自: 140.112.250.149 (04/24 00:37)