課程名稱︰分析化學丁
課程性質︰系定必修
課程教師︰劉春櫻
開課學院：醫學院
開課系所︰醫學檢驗暨生物技術學系
考試日期（年月日）︰2010/4/19
考試時限（分鐘）：120分鐘
是否需發放獎勵金：是
（如未明確表示，則不予發放）
試題 :
1.Explain the difference between (18%)
(a)precision and accuracy
(b)internal standard and standard addition
(c)sample mean and population mean
(d)random and determinate error
(e)a colloidal and a crystalline precipitate
(f)limits of detection and limits of quantitation
2.What is essential to state in your lab notebook? (3%)
3.State why standard additions and internal standards, instead of calibration
curve, are desirable, and why?
How to choose an appropriate internal standard? (8%)
4.How is a control chart used?State six indications that a process is going
out of control. (10%)
5.A method for the analysis of codeine in prescription drugs yielded the
following results when applied to a codeine free blank:0.1 , -0.2 , 0.3 ,
0.2 , 0.0 , -0.1 mg codeine. Calculate the detection limit(in terms of
milligrams of codeine) at the 99% confidence level, based upon the mean
of six analyses. (10%)
6.The homogemeity of a standard chloride sample was tested by analyzing
portions of the material from the top and the bottom pf the container, with
the following results:
% Chloride
Top Bottom
______________________________________________________________________________
26.32 26.28
26.33 26.25
26.38 26.38
26.39
______________________________________________________________________________
(a)Is nonhomogeneity indicated at the 95% confidence level?
(b)Is nonhomegeneity indicated at the 95% confidence level if it is known
that s->σ=0.03% Cl? (10%)
7.Calculate the titration error for the determination 40.00ml of 0.0502M KI
plus 0.0500M KBr titration with 0.0845M AgNO3.
(ksp for AgI is 8.3*10^-17 ; Ksp for AgBr is 5.0*10^-13) (8%)
8.Consider the solution prepared by
(a)dissolving 8.00mmol of NaOAc in 200ml of 0.100M HOAc.
(b)adding 100ml of 0.0500M NaOH to 100ml of0.175M HOAc.
(c)adding 40.0ml of 0.1200M HCl to 160.0ml of 0.0420M NaOAc.
In what respect do these solutions resemble one another?
How do they differ? (12%)
9.Give all the chemical equations to state the method for the determination
the protein content in the milk with Kjeldahl method. (12%)
10.(a)Explain the terms of isoionic point and isoelectric point.
(b)Please calculate the isoionic point and isoelectric point of 1.0M
alanine, based on the equations you derive.(For alanine:Ka1=4.53*10^-3 ;
Ka2=1.36*10^-10) (14%)
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