課程名稱︰分析化學乙上
課程性質︰生技系必修
課程教師︰劉春櫻
開課學院：理學院
開課系所︰化學系
考試日期（年月日）︰2010/11/12
考試時限（分鐘）：130分鐘
是否需發放獎勵金：是
（如未明確表示，則不予發放）
試題
1. Explain the difference between (32%)
(a) constant and proportional error
(b) random and systematic error
(c) confidence interval and confidence limit
(d) limit of detection and limit of quantitation
(e) peptization and coagulation of a colloid
(f) nucleation and partical growth
(g) digestion and extration
(h) internal standard and standard addition
2.Why are the standard reagents used in neutralization titrations generally
strong acids and bases rather than weal acids amd bases? (3%)
3.What factors affect end-point sharpness in an (a) acid/base titration?
(b) precipitation titration? (4%)
4.Please give the equations to describe how to determine chloride ion by
the Volhard method. Why does a Volhard determination of iodide ion require
fewer steps than a Volhard determination of chloride ion? (5%)
5.(a) Would you need NaOH or HCl to bring the pH of 0.050M HEPES to 7.45?
(b) Describe how to prepare 0.250L of 0.050M HEPES, pH 7.45.
(pKa of HEPES=7.56) (5%)
6. Give an example to describe the method of precipitation from homogeneous
solution. What is the advantage of it by the comparison with the conventional
method? (5%)
7. Do you know how to determine pH inside a small tissue? (6%)
8. The following results were obtained for the determination of the ppm P in
blood serum: 4.40, 4.42, 4.60, 4.48, 4.50. Determine whether the 4.60 ppm
result is an outlier or should be retained at the 95% confidence level. (6%)
9.What is the control chart? State whether or not the observations meet the
criteria for stability in a control chart. Please state the criteria first.(8%)
(附表)
10. Analysis of several plant-food preparations for potassium ion yielded the
following data:
Sample Percent K+
______________________________________
1 5.15 5.03 5.04 5.18 5.20
2 7.18 7.17 6.97
3 4.00 3.93 4.15 3.86
4 4.68 4.85 4.79 4.62
5 6.04 6.02 5.82 6.06 5.88
_______________________________________
The preparations were randomly drown from the same population.
(a) Find the mean and standard deviation s for each sample.
(b) Obtain the pooled standard deviation.
(c) Why is this a better estimate δ than the standard deviation from any one
sample? (12%)
11. A series of sequential baseline absorbance measurements are made in a
spectrophotometric method, for determining the purity of aspirins in tablets
using a blank solution. The absorbance readings are 0.002, 0.000, 0.008,
0.006, and 0.003. A standard 1 ppm aspirin solution gives an absorbance
reading of 0.051. What is the concentration detection limit at the 95%
confidence level of the method for the mean od five analysis. (14%)
附表--
Critical values of G for rejection od outlier
Number of observations G(95% confidence)
________________________________________________
4 1.463
5 1.672
6 1.822
7 1.938
8 2.032
9 2.110
10 2.176
11 2.234
12 2.285
15 2.409
20 2.557
_______________________________________________
附表Student's t
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※ 編輯: WilsonSun 來自: 140.112.252.220 (11/12 14:56)