課程名稱︰分子生物學
課程性質︰選修
課程教師︰張俊哲 老師
開課系所︰生物資訊學程
考試時間︰2006/5/12 上午
試題 :
1. Write down respective full name in English for the following abbreviations:
(1) DNA; (2) tRNA; (3) PCR; (4) RT-PCR; (5) UTR (5%)
2. Explain what is the "semi-conservative mode" for DNA replication. (5%)
3. Briefly describe:
(1) the universal features of a DNA replication fork. (6%)
(2) the differences in the numbers of DNA-replication origins between prokaryotes with circular genome and eukaryotes with linear genome. (3%)
(3) the differences in the speed of DNA-replication between prokaryotes and eukaryotes. (3%)
(4) How okazaki fragments are linked together in the lagging strand. (3%)
4. Describe how telomerase maintain the length of genome during DNA replication. (5%)
5. Do you agree with that the RNA polymerase II only has the function for synthesizing mRNA? If "Yes," write down how it performs transcription. If "No," write down other functions of this enzyme and how it works. (5%)
6. Compare the differences of "transcription initiation" between prokaryotes and eukaryotes, and write down at least two different points.(10%)
7. Briefly describe: (1) basic structure of a tryptophan operon; (2) how a tryptophan operon works under the presence of tryptophan (10%)
8. Explain/Describe: (1) why ribosomes are ribozymes; (2) the function of the nucleolus. (10%)
9. Write down where the following events occur in a eukaryotic cell: (1) DNA synthesis; (2) RNA synthesis; (3) the assembly of ribosomes; (4) protein synthesis; (5) RNA processing. (10%)
10.Describe how pre-mRNAs are processed to become mature in a eukaryotic cell and why the processing process is required. (10%)
11.Draw a schematic illustration of a transposable element of bacteria and briefly explain how it is inserted to other places in the genome. (10%)
12.Explain why some enzymes in the machinery of DNA replication can distinguish which strand is newly synthesized with the mismatch base oairs and which is the template strand? (5%)
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