課程名稱︰分子生物學
課程性質︰生技學程
課程教師︰吳益群 周子賓 靳宗洛
開課系所︰生技中心
考試時間︰2001.05.02
(此份試題為靳宗洛老師上課的部分)
試題 :
20 point2 of each , choice 5 questions
1.Transcription is catalyted by RNA polymerase with four stages.
a.Descibe functions of subunits of E.coli RNA polymerase.
b.Interaction RNA polymerase and DNA in different stages ,describe it.
2.Regulation of gene expression.
a.glucose and lactose metabolic regulation in Lac operon.
b.amino acids starvation induce stringent response.
c.tryptophan attenuate gene expression in Trp operon.
3.The machenism of lytic and lysogenic maintenance in lambda phage.
4.DNA replication
a.theta form, D-loop, and rolling circle in circular DNA replication.
b.the way to copy the end of DNA in linear DNA replication.
c.the mechanism of plasmid incompatibility.
5.DNA polymerase involves DNA replication.
a.functions of different types of DNA polymerase in E.coli.
b.the different molecule and its functin in replication fork.
6.DNA recombination and repair.
a.the mechanism of lambda phage DNA incorporate into bacterial
chromosome by intergration and excision.
b.the mismatch amd Uvr repair system.
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