課程名稱︰分子生物學
課程性質︰生技學程
課程教師︰靳宗洛 周子賓 吳益群
開課系所︰生技中心
考試時間︰2003.04.23
(此試題為靳宗洛老師上課的部份)
試題 :
(A) 30 points(2 for each)
1.sigma fortor
2.intrinsic terminator
3. polycistron
4.catabolite repression
5.ppGpp
6.siRNA
7.Episome
8.nut site
9.ter site and Tus
10.Okazaki fragment
11.relaxase
12.license factor
13.nick translation
14.Decatenation
15.Uvr repair system
(B) 20 points
a.Funstionc of different types DNA polymerase in E.coli
b.difference between Ori C and Ori Lambda replication initiation
in vitro
c.different molecules and their exactly function in primosome ,
replisome(replication fork) at Ori C and in termination site
(C) 20 points
a.Which three conditions needed at the recombination system in
bacteria to assimilate single-strnaded DNA
b.Assimilation involve with (1).chi site (2).Rec BCD complex (3).
other Rec factor (4) Ruv ABC factors
please to describe the mechanism
(D) 20 points
a.Difference between gene expression negative control by induction
and positive control by induction .
Give each one example and describe it.
b.E. coli cell when bearing with aλprophage is "immune" to lytic infection
by anotherλ, explain why ???
(E) 20 points
a.Please to describe how footprinting technique can used to identify
DNA binding site
b.(1) substrate and (2)Direction and (3) speed and (4)location for
replication , transcription and translation
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