[試題] 蛋白質結構與功能

[enisx (Tuor Oronar)]

課程名稱︰蛋白質結構與功能
課程性質︰選修
課程教師︰張文章
開課系所︰生化科學所
考試時間︰2002.11.12 期中考

試題 :

1.For thepeptide QMFENGCQGHCSERTFLLVH  (32%)
  What are the 4 possible reactions that can occur without enzymeatic
  catalysis? For each reaction the answer should include the following:

  i. the reaction and the product
 ii. the mass change due to the reaction
iii. the method to detect the reaction
 iv. the method to seprate the reactants and the products

2.Is it possible to detect the peptide in Question 1 by using the
  absorbance at 280nm? Why? (2%)

3.What would be the length of alpha-helix formed from the peptide in
  Question 1 ? Show your calculation. (4%)

4.For the enzymes containing alpha/beta domains the active sites are
  in a very similar position.Where are these active sites? (4%)

5.Describe Anfinsen's experment on ribonuclease A and its implication.
  (6%)

6.Explain why globuar proteins are only marginally stable by considering
  the thermodynamic factors deltaH and deltaS between the denatured and
  native stats. (10%)

7.Label the following 4 structures with cis or trans to indicate the
  peptide configurations (4%)
                                 Pro
R1   H       R1     R2     R1   / \       R1
 |_ _|_       |_ _ _|       |_ _\_/        |_ _/ \
   |   |        | |           |              | \_/
   O   R2       O H           O              O

8.Fill in each blank in the following with a suitable term or number
  (2% each)

@.The alpha helix contains a dipole moment aligned along the ( ) axis
  with partial positive charge at the ( ) terminus.

@.The hydrogen bonds in an alpha-helix are formed between the NH of the ith
  residue and the C=O of the  ( )th residue.

@.Most side chains in a transmembrane alpha-helix are (  )

@.Beta sheets have their beta-strands either (  ) or ( )

@.The EF hands contain 2 (   ) and a (  )

@.Two alpha-helices can packed against each other according to two models
  (   )  and  (  )

@.Hemagglutinin of influenza virus undergos a dramatic conformational
  changes whe its ( ) is lowered in the endosome.

@.Sickle-cell hemoglobin is formed fro the normal one by a mutation of
  (  aa) to  (  aa) in the 6th residue of the beta chain

@.The molten globule state is the intermediate in protein folding which
  contains most of the (  ) structure of the native state.
  A propeller-blade motif is formed by (   ) strands of anti-parallel beta
  strands.
  Serpins can inhibit the activity of (  )
  Calmodulin can bind ( ) ions and undergo a large (  )change on binding
  with its peptide ligands.
  The major secondary structure in proteins of the globin family is
  (  )

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期末考部分：
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※ 編輯: enisx           來自: 140.112.25.191       (11/17 17:40)