課程名稱︰細胞生物學實驗
課程性質︰必選
課程教師︰潘建源等教授
開課學院：生命科學院
開課系所︰生命科學系
考試日期（年月日）︰
考試時限（分鐘）：
是否需發放獎勵金：
（如未明確表示，則不予發放）
試題 :
941細胞生物學實驗期末考 潘老師部份
1. 單選題(5分)
(1)___ Which of the following statements is true?
a) Rhodamine 123 stains mitochondria and emits red light.
b) DiOC6 is specific for ER staining.
c) Mitotracker stains mitochondria by its thiol-reactive chloromethyl moiety.
d) Hoechst binds GC regions of nucleic acids and emits blue fluorescence.
(2)___ About chromaffin cells and rat brain cells,
a) chromaffin cells link well to each other by their neurites.
b) they are neuronal cell lines and can be passaged for a few times.
c) a nuclear region can be more than 1/4 of a cell soma in volume.
d) chromaffin cells are isolated from adrenal cortex.
(3) Choose a fit filter set for each dye:
__ (a) Hoechst 33258
__ (b) Rhodamine phalloidin
__ (c) Rhodamine 123
2. 簡答題(2分)
▲Give a technique used in 4-D imaging and explain how it works?
3. 思考與討論題(3分)
▲此實驗設計與我們胞器染色實驗相似，但採用流式細胞儀做記錄。請
根據此圖討論並解釋造成此實驗結果的原因。
Valinomycin FCCP
▲回答問題時，請參考這兩種藥物的化學式。
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