課程名稱︰分析化學乙下
課程性質︰一學年課程，一學期二學分
課程教師︰林萬寅
開課學院：生農學院
開課系所︰農業化學系
考試日期（年月日）︰06/20/2007
考試時限（分鐘）：120分鐘
是否需發放獎勵金：
（如未明確表示，則不予發放） 是
試題 :
1.Compound A and B were seperated with a 10-m GC column. The column has an
inner diameter of 0.25 mm and its wall is coated with 0.25-micro meter of
stationary phase.The retention times (tr) of the two compounds are given
below.It the retention time of the mobile phase(tm) is 2.0 min,answer the
following questions. 20%
Compound tr (min) W1/2
───────────────────────────
A 12.0 0.10
B 13.0 0.12
───────────────────────────
(a)Find the capacity factor (k') of both compounds.
(b)Find w (base width) and σ(standard deviation) for peak A.
(c)Find the number of theoretical plates and the plate height for compound A.
(d)Find the resolution and relative retention for compound A and B.
(e)Find the partition coefficient (K) for compound A.
2. An electrospray mass spectrum of the α chain of hemoglobin exhibits two
neighboring peaks with m/z=834.3 and 797.1,respectively.Estimate the charge of
each peak and the molar mass of the α chain of hemoglobin.(H=1.008) 10%
3.The interference mirror of a FT-IR spectrometer travels ±2cm.
(a)Explain how FT-IR works?
(b)Calculate the maximum retardation,Δ.
(c)Estimate the resolution (cm-1) of the instrucment.
(d)At what retardation intervel,δ,must the interferrogram be sampled to cover
a spectral range of 0-4000 cm-1 .
5.The natural abundance of carbon,chlorine,and bromine are listed below:
┌────┬────┬────┬────┬────┬────┐
│ C-12 │ C-13 │ Cl-35 │ Cl-37 │ Br-79 │ Br-81 │
├────┼────┼────┼────┼────┼────┤
│ 98.93% │ 1.07% │ 75.78% │ 24.22% │ 50.69% │ 49.31% │
└────┴────┴────┴────┴────┴────┘
Answer the following questions. 12%
(a)Find the normal mass(M) of C2Cl2Br2.
(b)List the possible combination of isotopes that lead to mass M+2 for
C2Cl2Br2.
(c)Find the relative peak intensities for (M+2)/M for C2Cl2Br2.
6.Answer the following questions. 48%
(a) Explain what is meant by chemical and isobaric interferences.
(b) Explain how the beam-chopping and Zeeman correlation work?
(c) Explain the different wall-coated,support-coated,and porouslayer open
tubular columns for GC.
(d) Explain how the hollow-cathode lamp works?
(e) List the variables that lead to band-broadening in chromatography.
(f) List the variables that contribute to the bandwidth in spectroscopy.
(g) Explain how the ion-exchange and molecular exclusion chromatography work?
(h) The weak base B (Kb=1.0×10^-5) equilibrates between water (phase1) and
benzene (phase2).Calculate the distribution coefficient D at pH 8.0 if the
partition coefficient K is 20.
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