課程名稱︰ 普通微生物學
課程性質︰ 微生物
課程教師︰ 林乃君負責期中前，期中後由吳慧芬老師授課
開課系所︰ 農化系必修 3學分
考試時間︰ 06年 4/23
試題 :
Midterm Exam
General Biology
1. Multiple choice (20 pts.)
(1) What component can not be found in bacterial membrane?
(a) sterol
(b) hopanoid
(c) phospholipid
(d) glycoprotein
(2) Rubisco (ribulose 1,5-bisphosphate carboxylase) is an abundant enzyme
invlolved in
(a) pentose phosphate pathway
(b) Entner-Doudoroff pathway
(c) glycolysis
(d) Calvin Cycle
(3) __________ means the first two positions of each codon are sufficient to
establish hydrogen bonding between the mRNA and tRNA, so it reduces the
need for a unique tRNA for each codon.
(a) code degeneracy
(b) wobble
(c) nonsense codon
(d) code redundancy
(4) __________ reaction is used to replenish the intermediates in TCA cycle
used in biosynthesis.
(a) amphibolic
(b) anaplerotic
(c) antiparallel
(d) anabolic
(5) After gram-stain procedure, gram-positive strain becomes purple (crystal
violet) because
(a) safarin can not stain it
(b) constriction of the thick cell wall prevents loss of crystal violets.
(c) the thick cell wall prevents entry of decolorizer such as ethanol or
acteone
(d) all of the above
(6) Thymine dimers cannot be repaired by
(a) photoreactivation
(b) excision repair
(c) recombination repair
(d) all of the above can repair thymine dimers
(7) Which description is correct?
(a) In Hfr x F- mating, the donor becomes F+ and the recipient becomes F+
(b) Tobacco mosaic virus is a helical virus which contains double-stranded
DNA genome
(c) In eukaryotes, mRNA synthesis is catalyzed by RNA polymerase II
(d) Eukaryotes use N- formylmethionine while prokaryotes use methionine
as initiator amino acid for protein synthesis
(8) Which one is not the function of the molecular chaperone?
(a) protect cell from environmental stress
(b) aid in protein folding
(c) transport proteins across the membrane
(d) all of the above are functions of the molecular chaperone.
(9) Who developed a postulate which established the link between a particular
microorganism and a particular disease?
(a) Robert Koch
(b) Louis Pasteur
(c) Edward Jenner
(d) Antony van Leeuwenhoek
(10) Which process does not need ATP?
(a) translation elongation
(b) move water through porin
(c) glycolysis
(d) gluconeogenesis
2. Fill in the black (20 pts.)
(1) Bacteria can be divided into several categories based on the energy
sources, electron/proton donor and carbon sources they utilize. _________
use light, inorganic electron/proton donor and CO2 while
chemoorganoheterotrophs use _________ energy source, __________
electron/proton donor and carbon sources.
(2) During photosynthesis, light energy is trapped in ________ and _______,
which are then used to reduce carbon dioxide to form carbohydrates.
(3) Synthesis of peptidoglycan involves UDP derivatives and a lipid carrier,
_________, which transports the backbone units across the cell membrane.
(4) Prokaryotic flagella are composed of basal body, _______, and _______.
When the bacteria move forward attractans, their flagella rotate in ______
direction more, which tends to lower the frequency of tumbling.
(5) Some microorganisms use _________ to aid uptake of iron.
3. Please choose the corresponding answer from options (a) to (h). (12 pts.)
(a) ribosome (b) lysosome (c) proteasome (d) polysome (e) dictyosome
(f) carboxysome (g) chromosome (h) mesosome
(1) stakcs of Golgi cisternae
(2) fixation of CO2 during Calvin Cycle
(3) proteins are ubiquitinated and then degraded
(4) synthesis of secreted protein
(5) mRNA complexed with many ribosomes
(6) DNA form during cell division
4. Terms and definition. (20 pts.)
(1) CFU and PFU
(2) anoxygenic and oxygenic photosynthesis
(3) dissimilatory and assimilatory nitrate reduction
(4) phagocytosis and pinocytosis
(5) lytic and lysogenic cycle
5. Please draw a typical bacterial growth curve and briefly describe why curve
looks like this. (8 pts.)
6. This is the DNA structure of an E. coli gene encoding for protein and its
mRNA. Which one is the template stand and which one is the nontemplate
strand? (above or below) What are (a), (b), (c) and (d) and what are their
functions
(a) (b)
5' ├─▇─▇─┼─────┼─────────┼─────┼─────┤
3' ──────────────────────────────────
Promoter antileader coding region antitrailer (d)
↓↓
mRNA ───▇─┼──────────────┼────
(c)
7. What are metabolic and structural adaptations for extreme temperatures
have thermophiles made? (10 pts.)
8. During metabolism, inorganic phosphate can be incorporated into ATP via
several phosphorylation processes. What are these? How are they different
from each other? (10 pts.)
9. Please use lac operon as an example, briefly describe how catabolic
repression works in the presence of lactose. (hint: repressor, inducer,
operator, structural genes etc.) (10 pts.)
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