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課程名稱︰分析化學二
課程性質︰系定必修
課程教師︰劉春櫻
開課系所︰化學系
考試時間︰2006/3/30 8:20~10:00
試題 :
1.Define the following terms:(24%)
a)isobestic point b)aptamer
c)stroke shift d)quenching
e)nebulizer f)antibody
g)flow injection analysis h)chemiluminescence
2.What are the advantages and disadvantages of decresing monochromator slit
width?(3%)
3.Describe how charge coupled device can be used as an extremely sensitive
detector.(6%)
4.How to get the composition of a complex with the method of continous
variation?(8%)
5.Explain how a laser generates light. List important properties of laser
light.(8%)
6.How to measure the equilibrium constant with the Scatchard plot?(8%)
7.Describe the differences between the following and list any paticular
advantages possessed by one over the other:(15%)
a)phototubes and photomultiplier tubes
b)conventional and diode-array spectrophotometers
c)fluorescence immunoassay and time-resolved fluorescence immunoassay
8.(a)Explain the following figure in terms of each labeled words.
(b)Disgussed how the property of fluorescent species dependent on the
parameters of molecular structure, structure rigidity, temperature and
solvent effects.(20%)
圖請參考 Quantitive chemical analysis/Daniel C. Harris/6ed p.421 fugure 18-13
9.Explain the following figure:(8%)
圖請參考 Quantitive chemical analysis/Daniel C. Harris/6ed p.433 上圖
10.Here is an immunoassay for explosive such as trinitrotoluene(TNT).
1)A column is prepared with covalently bound antibodies to TNT.
Fluorescent-labeled TNT is passed through the column to saturate the
antibodies with labeled TNT. The column is washed excess solvent until no
fluorescent is detected at the outlet.
2)Air containing TNT vapors is drawn through a sampling device that
concentrates traces of the gaseous vapors into distilled water.
3)Aliquots of water from the sample sre injected into the antibody column.
The fluorescence intensity of the liquid exiting the column is
proportional to the concentration of TNT in the water from the sampler
over the range 20 to 1200 ng/ml.
Draw pictures showing the state of the column in step 1 and 3 and explain
how the detector works.(8%)
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