課程名稱︰微生物學
課程性質︰選必
課程教師︰何國傑
開課學院：生命科學院
開課系所︰生命科學系
考試日期（年月日）︰95.4.2
考試時限（分鐘）：兩節課
是否需發放獎勵金：是
（如未明確表示，則不予發放）
試題 :
I.Define the follewings(20%)
1.molecular chaperones
2.substrate level phosphorylation
3.excision repair
4.specialized transduction
5.cloning vectors
II.Answer the following questions(80%)
1.What sources of energy used by microorganisms?(6%)
2.Why a microorganism can carry out glycolysis and the TCA cycle also has
the pentose phosphate pathway?(6%)
3.Sometimes a point mutation could cause the change of phenotype and
sometimes it does not.List all the reasons you can.(6%)
4.Describe the regulation of gene expression of lac operon,including negative
and positive control systems.(6%)
5.Describe how bacterial genome can be mapped.(6%)
6.The genus and species name of a bacterium is "bacillus" and "thuringiensis",
respectively.Write the scientific name of this organism correctly.(3%)
7.Why does a confocal microscope provide better images of thick specimens than
the standard compound microscope?(3%)
8.What characteristic makes acid-fast bacteria resistant to acid-alcohol
treatment?(3%)
9.What is the resolving power of a microscope?How can resolving power be
increased?What is the numerial aperture(NA) of a microscope?(9%)
10.What is a pure culture?What are the two basic steps in obtaining a pure
culture?(9%)
11.What are the four types of the flagella,as determined by number and
arrangement?Describe what makes a flagellum turn.(8%)
12.Explain the different relationships these types of microorganisms have with
oxygen:obligate aerobe,facultative anaerobe,aerotolerant anaerobe,strict
anaerobe and microaerophile.What are the enzymes the obligate aerobe and
facultative anaerobes usually have to destroy superoxide radical and
hydrogen peroxide?(10%)
13.How do you determine whether a chemical agent is bacteriostatic or
bactericidal?(5%)
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