課程名稱︰生物反應器的應用與設計
課程性質︰選修
課程教師︰何志煌
開課學院：生農
開課系所︰生物機電系
考試日期（年月日）︰2011/5/6
考試時限（分鐘）：4小時
是否需發放獎勵金：是,謝謝
（如未明確表示，則不予發放）
試題 :631U7870 Mid-term exam
(open notes)but not books due9:10am 5/6/2011 test time:4hrs.
1.簡答(25%)
a)In a stirred-tank bioreactor system, what parts provide for radial mixing?
And what for good axial mixing?(5)
b)In characteristic enzymatic kinetics, the initial reaction rate is
proportional to substrate concentration, [S],when[S] is low, but independent
of [S] when [S] is high. What is the physical meaning behind?(5)
c)Why does it take longer to sterilize(by autoclaving) a medium of bigger
volume than that of smaller volume?(5)
d)For temperature control, a water jacket is often used for a 20-liter
bioreactor system, while a stainless steel coil would be required for a 1000
liter system. Why?(5)
e)The number of reations involved in cell culture growth is enormous and their
interactions are complex. Yet, Monod kinetics is just as simple as Michaelis-
Menten kinetics. Why?(5)
2.(15%)Growth of a batch culture is recorded as
time (day) ∣ 1 ∣ 2 ∣ 3 ∣ 4 ∣ 5 ∣ 6 ∣ 7 ∣ 8 ∣ 9 ∣ 10
packed cell ∣2.2∣2.4∣3.1∣4.0∣5.1∣6.6∣8.6∣11.1∣14.3∣18.5
volume (ml)
time (day) ∣ 11 ∣ 12 ∣ 13 ∣ 14 ∣ 15 ∣ 16 ∣ 17 ∣
packed cell ∣23.9∣30.0∣35.4∣37.1∣37.2∣37.0∣37.3∣
volume (ml)
What are the duratio of its lag phase and doubling time(time required to
double biomass)? On what day would you subculture?
3.(15%)In the "substrate-inhibition" instances (S) may bind to the
substrate-enzyme complex(ES), generating a non-productive complex(ESS), as
shown below
E + S ﹤﹦﹥ES dissociation constant = Ks
ES + S ﹤﹦﹥ESS dissociation constant = Kss
k
ES → E + P
Where E,S and P are enzyme, substrate and product, respectively.
(1) State your assuptions clearly. And then develop a kinetic expression for
the initial reation rate, r , as a function of substrate concentration,[S]
(2) Sketch qualitatively the r v.s. [S] plot for the substrate-inhition
scenario.
4.(15%) Given: a bioreator of volume V; a cell suspention culture, which
showed growth characteristics as follows in a batch process.
time (day) ∣ 1 ∣ 2 ∣ 3 ∣ 4 ∣ 5 ∣ 6 ∣ 7 ∣ 8 ∣ 9 ∣ 10
packed cell ∣2.2∣3.1∣4.0∣5.1∣6.6∣8.6∣8.8∣8.9∣9.1∣8.9
volume (ml)
Objective: to maximize biomass productivity.
Assume: 1-day down-time between batches.
(1) Compare the operation efficiencis of a contunuous process and a batch
process.(10)
(2) What are the assumptions required in your comparison?(5)
5.(20%) In a CSTR , if cell death (=Kd X) is considered in the biomass growth
kinetics.
(1) What would be the design equations for X(biomass), P(product) and
S(substrate)? (10)
(2) What would be the wash-out dilution rate?(5)
(3) What would be steady-state biomass conc., Xss(5)
6.(10%) With a PFR of volume Vr, what is the washout dilution rate? Why?
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