課程名稱︰分子生物學
課程性質︰必修
課程教師︰靳宗洛
開課學院：生農學院、生科學院
開課系所︰
考試日期（年月日）︰2007.12.31
考試時限（分鐘）：120分鐘
是否需發放獎勵金：Yes
（如未明確表示，則不予發放）
試題 :
A: Term Explanation(2% of each),30%
1. IS 2. Nick translation 3.Intrinsic terminator
4. Topoisomerase 5. Photolyase 6. Chiasmata
7. Fts ring 8. Intrinsic terminator 9. Tus and ter site
10.T-DNA 11. Transposase 12. iDNA
13.Licensing factor 14. P elements 15. Eposome
B: Simple assay (5% of each), 50%
1. Describe 5 different DNA polymerases function in E.coli.
2. How Dam methylase in regulation of chromosome replication in E.coli?
3. How the Uvr repair system functioning in DNA excision repair systems?
4. DNA assimilation involves with (1) chi site (2) Rec complexes (3) Ruv
factors, please describe the mechanism.
5. What is "SOS response" and how to trigger it?
6. Describe MutT, M, Y system handles the consequences of oxidative damage.
7. What is Holliday junction and how to resolve it?
8. θ form, D-loop, and rolling circular in circular DNA replication and how
to copy the end of DNA in a linear DNA for replication.
9. Describe how to control of ColE1 plasmid incompatibility.
10. How to map the replication origin and quantitate of the replicons?
C: Assay(10% of each), 30%
1. How yeast to switch silent and active loci for mating type?
2. Molecules and each function in the primosome and replisome (in replication
fork) at Ori C, and how to resolve the catenation after replication.
3. Describe the conserved NHEJ mechanism for repairing in eukaryotic cells.
--
※ 發信站: 批踢踢實業坊(ptt.cc)
◆ From: 140.112.193.82
※ 編輯: sherrylo 來自: 140.112.193.82 (02/17 18:59)