課程名稱︰分析化學三
課程性質︰化學系必選修
課程教師︰陳俊顯
開課學院：理學院
開課系所︰化學系
考試日期︰96年10月29日
考試時限：10:20~12:15
是否需發放獎勵金：是
試題 :
1.(22%) (a) Write the full name of the following acronyms, and
(b) answer the question following the acronyms.
(7%)(1)HPLC; why does HPLC require a high pressure pump?
(8%)(2)SDS-PAGE; why does the separation of SDS-PAGE appear independent of
charge-to-size ratio of the analytes?
(7%)(3)FFF; draw a typical instrumentation setup of SFFF.
2.(30%) Answer the following questions in detail.
(一定要造出完整、明確的句子才會給分；只寫出片語、單字、不成句的表達，
則以零分計算。)
(9%)(1)Describe the "four solvent" method.
a.What does the "four solvents" mean?
b.How to use the nomographs?
c.How to optimize the solvent composition?
(11%)(2)Draw schemes of zeta potential versus distance from the capillart wall.
Use the two schemes to explain the reason why EOF is slower (2a) at a
lower pH and (2b) at a higher electrolytic concentration.
(10%)(3)Ion chromatography that separates cations.
a.What is the combination of the separation column and the suppressor
(i.e., what types of columns)?
b.Give an example to illustrate why the suppressor improve the signal
of of the sample zones?
3.(10%)Two compounds with partition coefficients of 0.12 and 0.18 are to be
separated on a column with V_M/V_S=3.00.
Calculate the number of theoretical plates needed to produce R_S=1.5.
4.(15%)Define the following terms.
(1) cold trapping (2) guard column (3) isoelectric point
6.(33%)(單選題，沒有正確選項時，打三角形，每題3分，選錯倒扣一分)
(1) Which of the following deos NOT lead to zone broadening in chromatography?
(a) Very high flow rates.
(b) Small column diameters.
(c) Very slow flow rates.
(2) Decreasing the thickness of the stationary phase leads to:
(a) decreased plate height, decreased retention time, and decreased
capacity for the analyte.
(b) decreased plate height, decreased retention time, and increased
capacity for the analyte.
(c) decreased plate height, increased retention time, and decreased
capacity for the analyte.
(3) Which of the following detector is a mass-sensitive detector?
(a) TCD.
(b) FID.
(c) ECD.
(4) Which of the following may lead to the possibility of both increase and
decrease the plate height of a column? (for GC)
(a) Increasing the weight of the stationary phase relative to the packing
weight.
(b) Decreasing the column temperature.
(c) Increasing the injection port temperature.
(5) Split-injection often used in capillary GC because it has
(a) a very sensitive detector.
(b) a large selectivity factor.
(c) a small capacity factor.
(6) Which of the following has the shortest t_R from a column containing a
reversed-phase packing?
(a) diethyl ether.
(b) n-hexane.
(c) benzene.
(7) Which of the following describes Sweeping better than the other two?
Analytes are concentrated by the collector
(a) due to analyte-collector interaction at the boundary between the sample
zone and the background electrolyte.
(b) because the collector and the analytes migrate in opposite directions.
(c) because the collector and the analytes migrate in the same directions.
(8) For dynamic SFE,
(a) the restrictor is always closed.
(b) the valve after the extraction cell is always open.
(c) the valve before the extraction cell is always close.
(9) Which of the following physical properties in NOT a typical(典型的)
gradient for SFC:
(a) temperature gradient.
(b) pressure gradient.
(c) polarity gradient.
(10) Which of the following electrophoretic methods separates sample zones
under the conditions that the zones have the same speed?
(a) CZE.
(b) CITP.
(c) CIEF.
(11) In FFFF, analytes with a larger molecular weight
(a) have a shorter retention time.
(b) have a larger l.
(c) are less permeable through the channel wall.
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