課程名稱︰生物化學甲上
課程性質︰必修
課程教師︰陳義雄 等人合開
開課學院：生命科學院
開課系所︰生化科學研究所
考試日期（年月日）︰
考試時限（分鐘）：
是否需發放獎勵金：是
（如未明確表示，則不予發放）
試題 :
1.Explain lactose but not sucrose is a reducing sugar(4%) and illustrate two
methods for the detection of a reducing sugar(2%)
2.Explain why oligosaccharides composed of three different monosaccharide
residues can produce a great variety of structural forms than oligopeptide
composed of three different amino acid residues does(6%)
3.Glycogen is soluble in water but it disperses readily in hot water to make a
turbid solution. Explain what is its structural features under the different
physical properties and the biological advantages of this physical property
(8%)
4.Briefly explain the depolarization and hyperpolarization of cell membrane
(4%), and describe how the light induces hyperpolarization of vision cells
to pass the signal to brain(8%)
5.Use the principle of cell signaling to explain how glucagon and insulin
regulate the blood level of glucose(8%)
6.Explain how cholera toxin and pertussis toxin cause the pathogenic effects
(8%)
7.What are the possible secondary messengers resulting from the response
termination of hormonal signals in G-protein-coupled system(5%)
8.Explain how p53 and Rb protein as the gene suppressors(6%)
9.What is physiological function of glycogen phosphorylase in skeletal muscle
and in liver, and why does the Vmax of this enzyme in muscle need to be
larger than that of the liver enzyme(6%)
10.Give the catalytic reactions of aldolase, dehydrogenase and mutase in the
glycolysis(6%)
11.Explain why the enzyme-limited reactions rather than the substrate-limited
reactions play the pivotal roles to regulate the flux of a metabolic pathway
(4%). Give the three enzyme-limited reactions as the control valve for the
glycolysis(6%). What are the effectors involved in the allosteric regulation
of phospho fructose kinase-1(4%)
12.If a small amount of [γ-32P]ATP is added to yeast extract, about half of
the 32P activity is formed in Pi within a few minutes, but the ATP
concentration remains unchanges. Explain(4%)
13.Give the factors affexting the direction of a biochemical reaction in cells
and why it is kinetically controlled(6%)
14.The standard reduction potentials of two half-reactions at 25℃ and pH 7 are
pyruvate + 2H+ + 2e- → lactate E'°(V)= -0.185
NAD+ + H+ + 2e- → NADH E'°(V)= -0.320
Give the oxidation and reduction when combined these two half reactions.
What is △G'°for the reaction at 25℃ (ln1.97=0.678) (8%)
15.What are the reaction steps involved in glycolysis with a net gain of 2ATP
and 2NADH from the oxidation of one glucose molecule to two pyruvate
molecules?(8%) How pyruvate is oxidized to recycle NADH to NAD+ which is
reutilized in the glycolysis?(6%)
16.What are main products of pentose phosphate pathway and what are their
functions?(6%)
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