課程名稱︰ 遺傳學
課程性質︰ 園藝系1 2 3 群必修
課程教師︰ 楊雯如 許圳塗
開課學院： 生農學院
開課系所︰ 園藝系
考試日期（年月日）︰2007.11.8
考試時限（分鐘）：120min
是否需發放獎勵金：是
（如未明確表示，則不予發放）
試題 :
九十六學年度遺傳學期中考題 2007/11/8
請依序作答
1. In the transformation experiment of Avery, McLeod, and McCarty, what was
the strongest evidence that the substance responsible for the transformation
was DNA rather than protein? (5%)
2. While study sewage, you discover a new type of bacteriophage that infects
E. coli. Chemical analysis reveals protein and RNA but no DNA. Is this
possible? (5%)
3. An synthetic mRNA molecule consists of the repeating base sequence
5'-AUCAUCAUCAUC AUCAUC…-3' . When this molecule is translated in vitro, the
result is a mixture of three polypeptide chains. One consists of repeating
isoleucines (Ile-Ile-Ile-…), another of repeating serines (Ser-Ser-Ser…),
and the third of repeating histidines (His-His-His…). What does this result
imply about the manner in which an mRNA is translated? (5%)
4. Red kernel color in wheat results from the presence of at least one
dominant allele of each of two independently segregating genes (in other
words, R-B- genotypes have red kernels). Kernels on rrbb plants are white,
and the genotypes R-bb and rrB- result in brown kernel color. Suppose that
plants of a variety that is true breeding for red kernels are crossed with
plants true breeding for white kernels. (10%)
(a). What is the expected phenotype of the F1 plants?
(b). What are the expected phenotypic classes in the F2 progeny and their
relative proportions?
5. Diagrammed here is DNA from a wildtype gene (top) and a mutant allele
(bottom) that has an insertion of a transposable element that inactivates the
genes. The symbols B and E represent the positions of restriction sites for
BamHI and EcoRI, respectively, and the rectangles show sites of hybridization
with each of three probes (A, B, and C) that are available. The dots at the
left indicated that the nearest site of either BamHI or EcoRI cleavage is
very far to the left of the region shown. Explain which probe and which
single restriction enzyme you would use for RFLP analysis to identify both
alleles. Also explain why any other choices would be unsuitable. (10%)
6. Complementation tests of the recessive mutant genes a through f produces
the data in the accompanying matrix. The circles represent missing data.
Assuming that all of the missing mutant combinations would yield data
consistent with the entries that are known, complete the table by filling
each circle with a+ or - as needed. (5%)
7. The diagrams shown here depict anaphase in cell division in a cell of a
hypothetical organism with one pair of chromosomes. Identify the panels as
being anaphase of mitosis, anaphase I of meiosis, and anaphase II of meiosis,
stating on what basis you reached your conclusions. (5%)
7. (a). 2n= 2A+xy代表什麼意思？(3%)
(b). 在蠅子草機制上和老鼠一樣，性別表現為male，請問機制為何？如何證明？(7%)
8. The accompanying diagram summarizes of the recombination frequencies
observed in a large experiment to study three linked genes.
What was the observed frequency of double crossing-over in this experiment?
Calculate the interfence. (10%)
9. In corn, the alleles C and c result in colored verses colorless seeds, Wx
and wx in non waxy verses waxy endosperm, and Sh and sh in plump verses
shrunken endpsperm. When plants grown from seeds heterozygous for each of
these pairs of alleles were testcrossed with plants from colorless waxy
shrunken seeds, the progeny seeds were as follows:
Colorless, nonwaxy, shrunken 84
Colorless, nonwaxy, plump 974
Colorless, waxy, shrunken 20
Colorless, waxy, plump 2349
Colorled, waxy, shrunken 951
Colorled, waxy, plump 99
Colorled, nonwaxy, shrunken 2216
Colorled, nonwaxy, plump 15
TOTAL 6708
Determine the order of the three genes, and construct a linkage map showing
the genetic distance between adjacent genes. (10%)
10. What is the chemical difference between the groups joined by DNA
polymerase and DNA ligase? (5%) Why can RNA polymerases and primases initiate
DNA replication, whereas DNA polymerase cannot? (5%)
11. Specific the functionor enzymatic activity of the following proteins that
participate in DNA replication: helicase, single stranded DNA binding
protein, gyrase, polymerase III, II, and I , DNA ligase, primerase. (10%)
12. 畫一個Gene expression 的示意圖，包含各種RNAs. (10%)
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