課程名稱︰生物工程導稐
課程性質︰選修
課程教師︰吳嘉文
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考試日期（年月日）︰期末
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試題 :
1.the modern discovery which disrupted the orginal central dogma is that flow
of genetic information is: replication transcription translation
2.what is the requirement for a template strand in DNA replication?
a.it serves as a guide in determining the next nucleotide to be
added according to the watson crick base pairing scheme.
b.it serves as the start point for the new DNA strand
c.it allows the DNA polymerase to move along it easily
d.it's a subsrate for the 3'-5' exonuclease activity.
3.all the following describe the general mechanism of DNA synthesis except
a.one strand is made 5'->3' while the other strand is made 3'->5'.
b.the strands become separated during synthesis.
c.synthesis occurs in both directions from the starting site of the synthesis.
d.synthesis of DNA is a very accurate process.
4.which of the following best describes the process of DNA replication in a
prokaryote ?
a.replication beings at a unique site and proceeds in one direction all the
way around a typically circular chromosome.
b.replication beings at a unique site and proceeds in two direction, meeting
aboout halfway around a typically circular chromosome.
c.replication beings at a multiple sites , spreading outward until the entire
typically circular chromosome is replicated.
d.replication beings at a multiple sites , spreading outward until the entire
typically linear chromosome is replicated.
5.the universal features of DNA replication include all the following, except
a.release of PPi from a nucleoside triphosphate.
b.synthesis from 5' end to 3' end.
c.base pairing of A to C and G to C.
d.use of a primer.
e.all of these
6.having the ability to degrade DNA allows DNA polymerases to perform their
job properly and efficiently.
true
7.the primer for in vivo DNA replication is :
a.the 3' hydroxyl of the preceeding Okazaki fragment.
b. a short piece of DNA.
c.a nick made in the DNA template.
d.a primer isn't always required for DNA replication.
e.all of these are true.
8.which of the following isn't a function of DNA polymerase I from E.coli?
a.adding nucleotides to the primer strand.
b.3'->5' exonuclease activity
c.5'->3' exonuclease activity
d.proofreading
9.E.coli replication on the lagging strand
a.us carried out by DNa polymerase 1
b.is initially synthesized as Okazaki fragment.
c.is synthesized continuously
d.has this DNA strand synthesized in a 3'->5' direction.
10.which of the following activities does E.coli DNA polymerase ?
a.5'->3' polymerase
b.5'->3' exonuclease
c.3'->5' exonuclease
d.E.coli DNA polymerase 3 jas all of above P.28
11.transcription of RNA and replication of DNA are similar in all these ways
except
a.nucleoside triphosphates are the precursors.
b.both strands of DNA are copied
c.base pairing is used to copy the sequence in a precise manner
d.the chain grows for the 5' to 3' end.
12.the universal features of DNA transcription include all the following except
a.release of PPi from a nucleoside triphosphate
b.synthesis from the 3'->5' end.
c.base pairing of A to U and G to C.
d.use of a primer.
13.the sigma subunit of RNA polymerase has all the following properties except
a.it tells the RNA polymerase where to sit down.
b.it helps point the RNA polymerase in the proper direction.
c.it causes the RNA polymerase to bind tightly to the DNA.
d.it stays with RNA polymerase throughout synthesis.
14.these terms can be used interchangeably to denote the complementary strand
in DNA except
a.template strand
b.coding strand
c.antisense strand
d.negative or "-"strand
15.chain termination occurs, in vivo, when :
a.RNA pol gets to the end of the DNA.本選項唬爛人
b.the factor called rho binds to the DNA.
c.a hairpin loop forms in the template.
d.either a hairpin loop forms or rho is involved
16.which of the following correctly describes a difference between RNA and DNA
polymerase?
a.RNA polymerases usually don't need a template while DNA polymerases do.
b.DNA polymerases usually require a primer, while most RNA
polymerases do not.
c.RNA polymerases usually synthesize introns, while DNA polymerases synthesize
cistrons.
d.RNA polymerases polymerize 5'->3', while DNA polymerases polymerize 3'->5'.
17.which of the following offers the best description of a Pribnow box?
a.a promoter consensus sequence located at approximately -35.
b.a promoter consensus sequence located at approximately -10.
c.a sequence forming a hairpin loop signaling the termination of transcription
d.a sequence intermediately surrounding the start site of transcription.
18.the promoter region is one term use to describe where RNA pol can bind to
DNA.
true
19.the DNA must form an open promter complex before RNA pol can bind it.
false after
20.the weakness of A-U base pairs at the end of the RNA molecule may help in
dissociation of the new RNA product.
true
21.the majority of protein synthesis occurs in the
a.nucleus
b.mitochondrion
c.cytoplasm老子錯了
d.nucleolus
22.as in RNA & DNA synthesis, the synthesis of proteins follows the following
steps except
a.activation of monomers
b.initiation
c.elongation
d.termination
e.all of these are involved in all polymer synthesis
23.the following terms describe the genetic code, except:
a.triplet
b.non-overlapping
c.commas are used
d.degenerate
24.degeneracy allows all of the following efficiencies in the genetic code,
except:
a.more than one amino acid can bind to a tRNA
b.each tRNA can bind to more than one codon.
c.most condons can bind to more than one tRNA
d.fewer tRNA molecules are needed.
25.all 64 of the codes for an amino acid
false
26.the wobble base is the 3' letter of the triplet codon.
true
27.which amino acids have unique codons?
a.met gly
b.trp met
c.tyr met
d.stop his
28.a tRNA was determined to have the following anticodon sequence
3'-GAI-5' (I represents the base hypoxanthine) indicate which of the following
codons can form base pairs with this anticodon
a.5'-CUA-3'
b.5'-CUC-3'
c.5'-CUU-3'
d.all of above
29.the genetic code is said to degenerate. this means that
a.each codon codes for more than one amino acid
b.each anticodon can interact with many different triplet sequences in the
mRNA, which may differ in any or all of the three nucleotides
c.many of the amino acids are coded for by different codons
d.the code is universally used by virtually all species
30.the process of amino acid activation
a.involves the formation of a peptide bond between the amino acid and tRNA
b.takes place by formation of a mixed anhydride of the amino acid and tRNA
c.involves the formation of an ester bond between the amino acid and tRNA
question
1.describe all the necessary process to get DNA from E.coli.
2.describe the filter-binding assay and how to use this method to determine
the relationships between codons and the corresponding amino acids.
explain
1.sticky ends
2.RNAi
3.del electrophoresis
4.northern blotting:
5.FISH(fluorescence in-situ hybridization)
6.EDTA
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