課程名稱︰生物化學概論
課程性質︰選修
課程教師︰丁一倪
開課系所︰化工系
考試時間︰94,6,23
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1.Maintaining restriction endonuclease resistence after DNA
replication is by
A.methylation C.acetylation
B.phosphorylation D.ethylation
2.What nuclease is reponsile for proofreading duing DNA replication?
A.3'→5' exonuclease C.3'→5' endonuclease
B.5'→3' exonuclease D.5'→3' endonuclease
3.When bacterial DNA replication introduces a mismatch in a double-
stranded DNA, the methyl-directed repair system:
A.cannot distinguish the template strand from the newly replicated
strand
B.changes both the template strand and the newly replicated strand
C.corrects the DNA strand that is methylated
D.corrects the mismatch bychanging the newly replicated strand
E.corrects the mismatch bychanging the replicated strand
4.The zinc finger motif is present in a number of DNA binding proteins.
The amino acid residues of a zinc finger protein coordinated to a
zinc ion are
A.His,Arg B.Arg,Cys C.His,Cys D.His,Arg,Cys
5.下列那一種與RNA 生合成無關？
A.Enhancer C.Cap binding protein
B.Promoter D.Transcription faction
6.The sigma factor (σ) of prokaryotic RNA polymers is part of the
core enzyme.which one of the following statement is not ture for
sigma factor?
A.It binds transiently to the core and directs the enzyme to specific
binding sites on the DNA.
B.It is released from the core enzyme after the first phosphodiester
bond has been formed.
C.It must be present for transcription to occur.
D.It specifically recognizes the promoter sites.
7.Which of the following statements about eukaryotic RNA synthesis is
correct ?
A.Polymerse I is required for mRNA synthesis.
B.α-amanitin can block polymerase I activity.
C.All three polymerase need the same cofactors for transcription.
D.Termination of transcription requires the protein ρ factor.
E.the primary mRNA transcript is inactive.
8.Which one of the following is not a basal transcriptional factor
for eukaryotic cells.
A.TFIIE C.GC box binding protein Sp1
B.TFIIH D.TATA box binding protein (TBP)
9.Which one of the following RNA has the highest percentage of modi-
fied bases ?
A.mRNA B.tRNA C.rRNA D.hnRNA
10.AZT used in the treatment of HIV infection, can interfere with a
protein encoded by HIV genetic material but not by muman genome.
AZT acts in HIV-infected cells by:
A.inhibiting reverse transcriptase.
B.blocking ATP production.
C.inhibiting RNA processing.
D.inhibiting RNA polymerase II,
E.blocking deoxynucleotide synthesis.
11.下列那一個化合物為最佳之ribozyme可進行splicing ?
A.AMP B.CMP C.GMP D.TMP
12.If the sequence of a segment of mRNA is 5'-GCAUUCUUAAAA-3',the DNA
sequence complementary to it is
A.5'-GCATTCTTAAAA-3' C.5'-TTTTAAGAATGC-3'
B.5'-CGTAAGAATTTT-3' D.5'-AAAATTCTTACG-3'
13.Which one of the RNA fragments can from a hairpin?
A.UACGAAGUUCGUA C.CACAAGGUCCGGA
B.UGCGCAGUCUGUG D.CACGGAGCUCCUG
14.有關原核細胞基因之轉錄及轉譯,下列敘述何者為非 ?
A.當基因轉變為mRNA可同時進行轉譯為蛋白質
B.所有的mRNA皆為monocistronic, 只能合成單一蛋白質
C.催化轉錄的酵素為DNA-dependent RNA polymerse
D.其Genetic code和真核生物細胞是一樣的
15.Which of the following statement is correct ?
A.The encoding information os protein on DNA must be continuous.
B.Transcription always starts at AUG codon.
C.Translation always starts at the beginning end of mRNA.
D.No processing reaction for all RNA transcripts.
E.None of the above
16.Degeneracy of the genetic code denotes the existence of:
A.codon consisting of 2 bases
B.multiple codons for a single amino acid
C.a given codon codes for different amino acids
D.codons that include one or more of the "unusual" bases.
17.Which of the following are features of the wobble hypothesis ?
A.A naturally occurring tRNA exists in yeast that can read both
arginine and lysine codons.
B.A tRNA can recogize only one condon.
C.The "wobble" occurs only in the first base of the anticodon.
D.Some tRNAs can recognize codons that specify two different
amino acids, if both are nonpolar.
E.The third base in a codon always forms a normal Watson-Crick base
pair.
18.Which of the following is true about the genetic code ?
A.All codons recognized by a given tRNA encode different amino acids.
B.Several different codons may encode the same amino acid.
C.It is absolutely identical in all living things.
D.The base in the middle position of the tRNA anticodon sometimes
permits "wobble" base pairing with 2 or 3 different codons.
E.The first position of the tRNA anticodon is always adenosine.
19.t-RNA 在蛋白質合成過程, 被稱為 "Adaptor"是因為
A.t-RNA 可根據蛋白質序列合成mRNA
B.t-RNA 可將50S 及30S ribosomal subunits結合在一起
C.t-RNA 可 "READ" mRNA序列, 同時又可攜帶特定的胺基酸
D.t-RNA 可被特定的aminoacyl tRNA synthetase "Recognize",形成charged
t-RNA
20.The t-RNAs have 4 base-paired stem.To which loop or stem amino acids
are charged ?
A.the T loop
B.the D loop
C.anticodon loop
D.the acceptor stem
21.有一t-RNA 之anticodon 為5'-GGU-3',其相對應之mRNA codon最可能是：
A.5'-ACU-3' C.5'-CUA-3'
B.5'-CCA-3' D.5'-UCA-3'
22.Which of the following statements about tRNA molecules is false ?
A.A,C,G and U are the bases present in the molecule.
B.Although composed of a single strand of RNA, each molecule con-
tains several short,double-helical regions.
C.Any given tRNA will accept only one specific amino acid.
D.There is at least one tRNA for each of the 20 amino acids.
E.The amino acid attachment is always to a C nucleotide at the 3'
end of the molecule.
23.In the "activation" of an amino acid for protein synthesis:
A.there is at least one specific activating enzyme and one specific
tRNA for each amino acid.
B.leucine can be attached to ｔＲＮＡphe, by the aminoacyl-tRNA
synthetase specific for leucine.
C.methionine is first formylated, then attached to a specific tRNA.
D.the amino acid is attached to the 5' end of the tRNA through a
phosphodiester bond.
E.two separate enzymes are required, one to form the amonoacyl
adenylate, the other to attach the amino acid to the tRNA.
24.已知真核細胞合rRNAs:r-28S,r-18S,r-5.8S,r-5S 共四種, 已知其三個形成
一個precursor 其三個為:
A.r-28S,r-18S 與r-5.8S C.r-28S,r-18S 與r-5S
B.r-28S,r-5.8S與r-5S D.r-18S,r-5.8S與r-5S
25.One isolated a full-length mRNA with 1,000 nucleotides which could
encode a protein with a molecular weight of:
A. 30,000 C.120,000
B. 60,000 D.240,000
26.Which is not used in post-translational modification for insulin
synthesis ?
A.nuclear localization
B.disulfide linkage
C.protease cleavage
D.folding
27.Which of the following statements about regulation of the lac operon
is true ?
A.Glucose in the growth medium does not affect the inducibility by
lactose.
B.Glucose in the growth medium increases the inducibility by lactose.
C.Glucose in the growth medium decreases the inducibility by lactose.
D.Its expression is regulated mainly at the level of translation.
E.The lac operon is fully induced whenever lactose is present.
28.IPTG (isopropylthiogalactoside) induction of β-galactosidase
activity is the result of
A.stimulation of Lac repressor function.
B.IPTG binding to the lac operon and inducing transcription.
C.IPTG binding to the lacI gene product and inhibiting its
activity.
29.Frederick Sanger developed the dideoxy method for DNA sequencing.
What is the principle that Sanger based on ?
A.hybridization
B.replication
C.transcription
D.restriction enzyme mapping
E.inhibition of β-galactoside degradation.
1.Which of the following statement about DNA replication is incorrect?
(1) DNA replication occurs at S phase
(2) In DNA replication DNA polymerase Ⅲ is used for polymerization
of leading strand and lagging strand.
(3) The direction of lagging strand DNA synthesis is opposite to
the direction of movement of replication fork.