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※ 引述《cypher0623 (爽爽爽爽爽爽爽爽爽爽爽)》之銘言: : 【問題】:請問人工胃/胰液的配法為何? : 因為小弟查到的資料中胃蛋白脢pepsin,胰蛋白脢trypsin的含量有好幾種說法, : 而且差異頗大,所以上版來問問.... : 我查到資料中光pepsin的使用量從10g~1mg/100ml都有....@_@ : 有人知道哪種才是比較恰當的配法嗎? : 亦或該使用酵素活性單位為配方依據呢?? 美國藥典有說: Gastric Fluid, Simulated, TS —Dissolve 2.0 g of sodium chloride and 3.2 g of purified pepsin,that is derived from porcine stomach mucosa, with an activity of 800 to 2500 units per mg of protein, in 7.0 mL of hydrochloric acid and sufficient water to make 1000 mL. [ NOTE—Pepsin activity is described in the Food Chemicals Codex specifications under General Tests and Assays.] This test solution has a pH of about 1.2 Intestinal Fluid, Simulated, TS —Dissolve 6.8 g of monobasic potassium phosphate in 250 mL of water, mix, and add 77 mL of 0.2 N sodium hydroxide and 500 mL of water. Add 10.0 g of pancreatin, mix, and adjust the resulting solution with either 0.2 N sodium hydroxide or 0.2 N hydrochloric acid to a pH of 6.8 ± 0.1. Dilute with water to 1000 mL. -- 織田信長:「杜鵑不叫,我殺了牠!」 豐臣秀吉:「杜鵑不叫,我想辦法讓牠叫!」 德川家康:「杜鵑不叫,我等牠叫!」 Angus Tzeng:「杜鵑不叫,我自己叫!XD」 -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 125.224.156.173
cypher0623:感謝F大 太感動了 T_____T 10/01 13:03