※ 引述《fjubiology (Science閱讀俱樂部)》之銘言:
: ※ 引述《cypher0623 (爽爽爽爽爽爽爽爽爽爽爽)》之銘言:
: : 【問題】:請問人工胃/胰液的配法為何?
: : 因為小弟查到的資料中胃蛋白脢pepsin,胰蛋白脢trypsin的含量有好幾種說法,
: : 而且差異頗大,所以上版來問問....
: : 我查到資料中光pepsin的使用量從10g~1mg/100ml都有....@_@
: : 有人知道哪種才是比較恰當的配法嗎?
: : 亦或該使用酵素活性單位為配方依據呢??
: 美國藥典有說:
: Gastric Fluid, Simulated, TS —Dissolve 2.0 g of sodium chloride and 3.2 g of
: purified pepsin,that is derived from porcine stomach mucosa, with an activity
: of 800 to 2500 units per mg of protein, in 7.0 mL of hydrochloric acid and
: sufficient water to make 1000 mL. [ NOTE—Pepsin activity is described in the
: Food Chemicals Codex specifications under General Tests and Assays.]
: This test solution has a pH of about 1.2
: Intestinal Fluid, Simulated, TS —Dissolve 6.8 g of monobasic potassium
: phosphate in 250 mL of water, mix, and add 77 mL of 0.2 N sodium hydroxide
: and 500 mL of water. Add 10.0 g of pancreatin, mix, and adjust the resulting
: solution with either 0.2 N sodium hydroxide or 0.2 N hydrochloric acid to a
: pH of 6.8 ± 0.1. Dilute with water to 1000 mL.
英國藥典有說:
Gastric Juice, Artificial
Dissolve 2.0 g of sodium chloride and 3.2 g of pepsin powder in water .
Add 80 ml of 1M hydrochloric acid and dilute to 1000 ml with water .
Pepsin
A substance containing a proteolytic enzyme of the gastric secretion of
animals, diluted, if necessary, by admixture with Lactose or Sucrose. Use a
grade of commerce capable of digesting 2500 times its own weight of
coagulated egg albumen.
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