A Novel Coronavirus Associated with Severe Acute Respiratory Syndrome.
Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret T, Emery S, Tong S,
Urbani C, Comer JA, Lim W, Rollin PE, Dowell SF, Ling AE, Humphrey CD,
Shieh WJ, Guarner J, Paddock CD, Rota P, Fields B, DeRisi J, Yang JY,
Cox N, Hughes JM, LeDuc JW, Bellini WJ, Anderson LJ.
Background A worldwide outbreak of severe acute respiratory syndrome (SARS)
has been associated with exposures originating from a single ill health care
worker from Guangdong Province, China. We conducted studies to identify the
etiologic agent of this outbreak. Methods We received clinical specimens
from patients in seven countries and tested them, using virus-isolation
techniques, electron-microscopical and histologic studies, and molecular
and serologic assays, in an attempt to identify a wide range of potential
pathogens.Results None of the previously described respiratory pathogens
were consistently identified. However, a novel coronavirus was isolated from
patients who met the case definition of SARS. Cytopathological features were
noted in Vero E6 cells inoculated with a throat-swab specimen. Electron-
microscopical examination revealed ultrastructural features characteristic
of coronaviruses. Immunohistochemical and immunofluorescence staining
revealed reactivity with group I coronavirus polyclonal antibodies.
Consensus coronavirus primers designed to amplify a fragment of the
polymerase gene by reverse transcription-polymerase chain reaction
(RT-PCR) were used to obtain a sequence that clearly identified the
isolate as a unique coronavirus only distantly related to previously
sequenced coronaviruses. With specific diagnostic RT-PCR primers we
identified several identical nucleotide sequences in 12 patients from
several locations, a finding consistent with a point-source outbreak.
Indirect fluorescence antibody tests and enzyme-linked immunosorbent
assays made with the new isolate have been used to demonstrate a virus-
specific serologic response. This virus may never before have circulated
in the U.S. population. Conclusions A novel coronavirus is associated
with this outbreak, and the evidence indicates that this virus has an
etiologic role in SARS. Because of the death of Dr. Carlo Urbani, we
propose that our first isolate be named the Urbani strain of SARS-
associated coronavirus.
Notice: Because of possible public health implications, this article was
published at www.nejm.org on April 10, 2003. It will appear in the May 15
issue of the Journal. Click on "PDF of this article" for the full text.
Copyright 2003 Massachusetts Medical Society （N Engl J Med 2003 Apr 30;
[epub ahead of print]）
變種冠狀病毒與 SARS相關
Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret T, Emery S, Tong S,
Urbani C, Comer JA, Lim W, Rollin PE, Dowell SF, Ling AE, Humphrey CD,
Shieh WJ, Guarner J, Paddock CD, Rota P, Fields B, DeRisi J, Yang JY,
Cox N, Hughes JM, LeDuc JW, Bellini WJ, Anderson LJ.
全球性的SARS疫情源於與中國廣東省一位醫護人員病例的暴露接觸。本報告進行了
病原鑑定的工作。 收集七個國家的臨床檢體，以病毒分離、電子顯微鏡、組織學、
分子生物與血清學技術加以分析。由符合SARS定義的病患體內分離出變種冠狀病毒
（coronavirus），將病患喉頭擦拭標本接種在Vero E6細胞中進行細胞病理學觀察
，電子顯微鏡顯示典型冠狀病毒微細結構特徵。免疫組織化學與免疫螢光染色顯示
此病毒可與group 1冠狀病毒抗體結合。以冠狀病毒聚合酵素保守序列設計的RT-PCR
引子（primer）進行反應，獲得的聚合酵素核酸片段序列顯示此變種病毒與以前所
定序的冠狀病毒極為不同。
以不同的RT-PCR引子，由不同地點的12位病患採得檢體中分別獲得數段相同的序列
；與在同一地點的不同患者結果一致。以間接螢光抗體及酵素結合免疫球吸附分析
（enzyme- linked immunosorbent assay）此新分離株，顯示出病毒特有的血清反
應。此病毒過去在美國民眾中可能未曾發生感染。
結論：一變種冠狀病毒與此次SARS疫情相關，並有證據顯示此病毒與SARS病因相關。
由於Dr. Carlo Urbani殉職，吾人將此病毒株命名為Urbani strain of SARS-related
coronavirus.（N Engl J Med 2003 Apr 30; [epub ahead of print]
注意：因本文之公共衛生意涵，本文內容已於4月10日先發表於www.nejm.org，全文將
於5月15日該刊中發表）
2003-05-15
Total Hits: 694
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嗯,好,這是1個月前的NEJM
這是國家衛生研究局的翻譯
http://sars.nhri.org.tw/taskforce2_1.php?indx=195&page_title=科學研究
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風は甘い蜜を運び偽りの影踴らせる
深い倒錯に觸れた恍惚の果てに溺れてゆく
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